Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for measuring dissolution rate of andrographolide pills

A technology of andrographolide, determination method, applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve a good linear effect

Active Publication Date: 2014-07-02
TIANJIN TASLY PHARMA CO LTD
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since dissolution and dissolution of a drug have a significant impact on absorption, in vitro dissolution testing has the potential to predict its in vivo behavior

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring dissolution rate of andrographolide pills
  • Method for measuring dissolution rate of andrographolide pills
  • Method for measuring dissolution rate of andrographolide pills

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] 1. Experimental materials

[0124] 1.1 Reagents and reagents

[0125] Andrographolide reference substance: China National Institute for the Control of Pharmaceutical and Biological Products Batch number: 110797-200307

[0126] Andrographolide Pills: Tasly Pharmaceutical Group Co., Ltd.

[0127] (Lot numbers: 120203, 120204, 120205, 120523)

[0128] Methanol: Tianjin Concord Technology Co., Ltd.

[0129] Ultrapure water: self-made by the company

[0130] 1.2 Instruments

[0131] Intelligent Drug Dissolution Apparatus: Tianda Tianfa Company Model: RCZ-8B

[0132] Dissolution sampling collection system: Tianfa Tianfa Company Model: RZQ-8D sampling collection system composition

[0133] High performance liquid chromatograph: American Waters Company Model: Waters 2965-2489

[0134] Column: Waters XBridge TM Shield RP18 (100×4.6mm, 3.5μm)

[0135] S / N: 01273132014001

[0136] 1.3 Chromatographic conditions

[0137] Mobile phase: methanol-water (volume ratio 50:50);...

Embodiment 2

[0143] 1. Experimental materials

[0144] With embodiment 1.

[0145] 1.2 Instruments

[0146] With embodiment 1.

[0147] 1.3 Chromatographic conditions

[0148] Mobile phase: methanol-water (volume ratio 40:60); column temperature: 40°C; flow rate: 0.8ml / min; detection wavelength: 225nm.

[0149] Determination of dissolution rate:

[0150] (1) Preparation of the reference substance solution: an appropriate amount of the reference substance of andrographolide, accurately weighed, add an appropriate amount of methanol to dissolve, quantitatively dilute with 0.5% sodium lauryl sulfate solution to prepare a solution containing about 0.13 mg of andrographolide per 1 ml solution, as a reference substance solution;

[0151] (2) Preparation of the test solution: get 1 bag of andrographolide dripping pills, adopt the slurry method to measure the dissolution rate of andrographolide dripping pills, take 0.5% sodium lauryl sulfate solution 1000ml as the dissolution medium, and the ...

Embodiment 3

[0154] 1. Experimental materials

[0155] With embodiment 1.

[0156] 1.2 Instruments

[0157] With embodiment 1.

[0158] 1.3 Chromatographic conditions

[0159] Mobile phase: methanol-water (volume ratio 60:40); column temperature: 30°C; flow rate: 1.2ml / min; detection wavelength: 225nm.

[0160] Determination of dissolution rate:

[0161] (1) Preparation of reference substance solution: an appropriate amount of andrographolide reference substance, accurately weighed, add an appropriate amount of methanol to dissolve, quantitatively dilute with 1% Brij solution to make a solution containing about 0.17mg of andrographolide per 1ml, as a reference substance solution;

[0162] (2) Preparation of test solution: get 1 bag of andrographolide dripping pills, adopt the slurry method to measure the dissolution rate of andrographolide dropping pills, take 1% Brij solution 1000ml as the dissolution medium, the rotating speed is 50 revolutions per minute, the temperature After 45 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for measuring the dissolution rate of andrographolide pills. The method comprises the following steps: preparing a reference substance solution, preparing a test solution, and then measuring the content of andrographolide in the test solution by using a high performance liquid chromatography, and comparing the content with a labeled amount to calculate the dissolution rate. The method is excellent in linearity, repeatability, reproducibility and recycling rate, and facilitates the relatively-overall control of quality of the andrographolide pills.

Description

Technical field: [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a detection method of traditional Chinese medicines, in particular to a method for measuring the dissolution rate of andrographolide dripping pills. Background technique: [0002] After oral administration of a solid dosage form, drug absorption depends on dissolution or release of the drug from the dosage form, dissolution of the drug under physiological conditions, and penetration in the gastrointestinal tract. Since dissolution and dissolution of a drug have a significant impact on absorption, in vitro dissolution testing has the potential to predict its in vivo behavior. Based on the above considerations, the establishment of an in vitro dissolution test method for oral solid dosage forms has the following functions: [0003] (1) Evaluate the consistency of the batch-to-batch quality of preparations; [0004] (2) Guide the research and development of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88
Inventor 边宁葛丹丹侯春莲孙玉侠曹凤兰
Owner TIANJIN TASLY PHARMA CO LTD