Preparation of Bacillus coagulans probiotics

A technology for Bacillus coagulans and probiotic preparations, which is applied in the application field of solid-state fermentation of probiotic Bacillus coagulans HM-09, can solve problems such as large environmental pollution, cumbersome operation of machinery and equipment, and difficult post-processing, and achieves increasing daily growth. Sensory characteristics such as weight, colour and mouthfeel and effects of improvement in sensory characteristics such as variety, colour and mouthfeel

Active Publication Date: 2016-08-10
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Bacillus coagulans HM-09 probiotic preparation, the preparation method of which includes strain culture and solid-state fermentation culture.

[0042] Bacillus coagulans HM-09 seed culture conditions, placed on a shaker, the speed is 170r / min, the temperature is 37°C, cultured for 24h;

[0043] The composition of Bacillus coagulans HM-09 seed medium is as follows: peptone 10.0g, yeast extract 10.0g, glucose 6.0g, MgSO 4 ·7H 2 O1.0g, K 2 HPO 4 2.0g, MnSO 4 50mg, add 1L distilled water, adjust the pH to 7.0-7.2;

[0044] The mass ratio of the solid-state fermentation medium is composed of soybean meal: bran: corn flour = 10:8:1, the water content is controlled at 60%, and it is autoclaved at 115°C for 25 minutes.

[0045] The inoculum amounts of Bacillus coagulans HM-09 were 2.0% (v / w), 5.0% (v / w) and 8.0% (v / w) respectively. The culture temperature was 37°C and the humidity was 70%. After 48 hours of solid-state fermentation of single bacteria The number...

Embodiment 2

[0047] Example 2: Bacillus coagulans HM-09 probiotic preparation, the preparation method of which includes strain culture and solid-state fermentation culture.

[0048] Bacillus coagulans HM-09 seed culture conditions, placed on a shaker, the speed is 170r / min, the temperature is 37°C, cultured for 24h;

[0049] The composition of Bacillus coagulans HM-09 seed medium is as follows: peptone 10.0g, yeast extract 10.0g, glucose 6.0g, MgSO 4 ·7H 2 O1.0g, K 2 HPO 4 2.0g, MnSO 4 50mg, add 1L distilled water, adjust the pH to 7.0-7.2;

[0050] Bifidobacterium animalis subsp. lactis V9 seeds were cultured at 37°C for 24 hours;

[0051] The composition of Bifidobacterium animalis subsp. lactis V9 seed culture medium is: 20g peptone, 20g glucose, add water to 1L, pH7.0-7.2.

[0052] The mass ratio of the solid-state fermentation medium is composed of soybean meal: bran: corn flour = 10:8:1, the water content is controlled at 60%, and it is autoclaved at 115°C for 25 minutes.

[0...

Embodiment 3

[0055] Embodiment 3: Bacillus coagulans HM-09 probiotics preparation, its preparation method comprises strain culture and its solid-state fermentation culture. Bacillus coagulans HM-09 seed culture conditions, placed on a shaker, the rotation speed is 160r / min, the temperature is 37°C, and cultured for 20h;

[0056] The composition of Bacillus coagulans HM-09 seed medium is as follows: 15.0g peptone, 15.0g yeast extract, 10.0g glucose, MgSO 4 ·7H 2 O1.0g, K 2 HPO 4 2.0g, MnSO 4 50mg, add 1L distilled water, adjust the pH to 7.0-7.2;

[0057] Bifidobacterium animalis subsp. lactis V9 seeds were cultured at 37°C for 20 hours;

[0058] The composition of Bifidobacterium animalis subsp. lactis V9 seed medium is: 50g peptone, 20g glucose, add water to 1L, pH7.0-7.2.

[0059] The mass ratio of the solid-state fermentation medium is composed of soybean meal: bran: corn flour = 10:10:0.5, the water content is controlled at 60%, and it is autoclaved at 115°C for 25 minutes.

[0...

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PUM

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Abstract

The invention relates to preparation of a Bacillus coagulans probiotic preparation, and belongs to the technical field of probiotic preparations. The probiotic preparation is prepared through solid-state fermentation, wherein the solid-state fermentation culture method comprises the steps of firstly inoculating Bacillus coagulans HM-09 in a ratio of 2.0-8.0% (v / w), fermenting at the temperature of 32-42 DEG C and humidity of 70-85% until pH decreases to 6.5-6.8, inoculating bifidobacterium lactis V9 in a ratio of 3.0-5.0% (v / w), fermenting at the temperature of 32-37 DEG C and humidity of 70-80%, carrying out mixed culture for 24-48h and then carrying out fluidized bed drying; the Bacillus coagulans HM-09 has the preservation number of CGMCC No.6729; the bifidobacterium lactis V9 has the preservation number of CGMCC No.5470. The preparation of the Bacillus coagulans probiotic preparation has the advantages that a staged inoculation technique is adopted, mixed bacterium fermentation has mutual promoting action on strain growth and proliferation, the color, mouthfeel and other sensing characteristics of the product obtained after fermentation are improved and diversified, and the degradation rate of nutrient substances is improved at the same time.

Description

technical field [0001] The invention belongs to the technical field of probiotic preparations, and relates to an application technology of solid-state fermentation with probiotic Bacillus coagulans HM-09 (Bacillus coagulans HM-09) using soybean meal, bran, and corn flour as substrates. This technology can be widely used in Animal probiotic microecology and other aspects. Background technique [0002] At present, probiotic products mostly use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, and the rest are fermented in liquid state to directly collect probiotic cells and make probiotic powder, tablets or capsules. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. Therefore, the present application adopts bacillus coagulans HM-09 (Bacillus coagulans HM-09) for solid-state f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01C12R1/07
Inventor 张善亭高鹏飞赵树平
Owner INNER MONGOLIA SCI PLUS BIOTECH
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