A kind of enzyme-linked immunochromogenic substrate and preparation method thereof
A chromogenic substrate and enzyme-linked immunosorbent technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of high operational accuracy, short storage period, difficulty in comparison and verification of test data, and achieve excellent stability. , reduce the difference between batches, and simplify the detection operation steps
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Embodiment 1
[0025] Add 1.0 g of citric acid monohydrate and 26 g of disodium hydrogen phosphate dodecahydrate to 800 mL of distilled water, adjust the pH to 4.0 with hydrochloric acid with a concentration of 6 M, and add distilled water to make the volume to 1000 mL to prepare a buffer mother solution; add 3.2 g tetramethylbenzidine hydrochloride and 0.2 g disodium edetate were stirred and dissolved to obtain a mixed solution; 15 mL glycerol, 98 mg sodium pyrophosphate and 9 mg sodium stannate were added to the mixed solution to avoid light Stir overnight to obtain a chromogenic substrate.
Embodiment 2
[0027] Add 1.5g of citric acid monohydrate and 30g of disodium hydrogen phosphate dodecahydrate to 800mL of distilled water, adjust the pH to 4.5 with hydrochloric acid with a concentration of 6M, add distilled water to make up to 1000mL to prepare buffer mother liquor; add 3.5 g of tetramethylbenzidine hydrochloride and 0.3 g of disodium edetate were stirred and dissolved to obtain a mixed solution; 20 mL of glycerol, 100 mg of sodium pyrophosphate and 10 mg of sodium stannate were added to the mixed solution to protect from light Stir overnight to obtain a chromogenic substrate.
Embodiment 3
[0029] Add 1.2g of citric acid monohydrate and 28g of disodium hydrogen phosphate dodecahydrate to 800mL of distilled water, adjust the pH to 4.2 with hydrochloric acid with a concentration of 6M, add distilled water to make the buffer mother solution to 1000mL; add 3.4 g of tetramethylbenzidine hydrochloride and 0.25 g of disodium edetate were stirred and dissolved to obtain a mixed solution; 17 mL of glycerol, 99 mg of sodium pyrophosphate and 10 mg of sodium stannate were added to the mixed solution to avoid light Stir overnight to obtain a chromogenic substrate.
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