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Novel application of nano-structure hollow calcium carbonate microsphere

A nano-structured, hollow microsphere technology, applied in the direction of being fixed on or in an inorganic carrier, can solve the problems of long production process cycle, large equipment investment, etc. Effect

Active Publication Date: 2014-07-16
靖江市城中村投资建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microencapsulation technology of microorganisms, especially microencapsulation technology of probiotics can solve the survival rate of probiotics very well. At present, there are many studies on the survival rate of probiotics, and microencapsulation freeze-drying currently has the highest survival rate, but its disadvantages It is a large investment in equipment and a long production process cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of lactic acid bacteria microcapsules

[0018] Inoculate the lactic acid bacteria seed liquid with 3% inoculation amount, culture at 37°C and 120r / min for 10h, centrifuge at 8000r / min for 5 minutes, add nanostructured calcium carbonate hollow microspheres accounting for 1% of the total weight of microorganisms, and nanostructured calcium carbonate hollow microspheres The ball size is 2μm, the wall thickness is 20nm, and the high-speed homogenizer is mixed to obtain the core material; 4% sodium alginate and 2% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled to After room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:2.5, pass through the Equl microcapsule generator, and add 7 g / l CaCl dropwise 2 , solidified for 35 minutes, and filtered to collect lactic acid bacteria microcapsules.

...

Embodiment 2

[0020] The preparation of embodiment 2 butyric acid bacteria microcapsules

[0021] Inoculate the butyric acid bacteria seed solution with 3% inoculation amount, culture at 37°C and 120r / min for 10h, centrifuge at 8000r / min for 5 minutes, add nanostructured calcium carbonate hollow microspheres accounting for 3% of the total weight of microorganisms, and nanostructured calcium carbonate hollow microspheres The microsphere size is 4μm, the wall thickness is 500nm, and the high-speed homogenizer is mixed to obtain the core material; 4% sodium alginate and 2% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled. After reaching room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:3.5, pass through the Equl microcapsule generator, and add 10g / l CaCl dropwise 2 , solidified for 50 minutes, and filtered to collect butyric acid bacteria mi...

Embodiment 3

[0023] Example 3 Preparation of Enterococcus faecium microcapsules

[0024] Inoculate the seed solution of Enterococcus faecium at 3% inoculum, culture at 37°C and 120r / min for 10h, centrifuge at 8000r / min for 5 minutes, add nanostructured calcium carbonate hollow microspheres accounting for 4% of the total weight of microorganisms, and nanostructured calcium carbonate Hollow microspheres with a size of 3 μm and a wall thickness of 200 nm were mixed with a high-speed homogenizer to obtain a core material; 4% sodium alginate and 2% gelatin were added to distilled water and mixed, and sterilized by high pressure to obtain an embedding agent. After cooling to room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:3, pass through the Equl microcapsule generator, and add 8g / l CaCl dropwise 2 , solidified for 40 minutes, and collected Enterococcus faecium microcapsules by filtration.

[0025]

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PUM

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Abstract

The invention discloses an application of a nano-structure hollow calcium carbonate microsphere in microbe microencapsulated culture. The invention also discloses a microbe microcapsule which contains the nano-structure hollow calcium carbonate microsphere accounting for 1%-8% of the total weight of microbes. The invention also discloses a preparation method of the microbe microcapsule. The invention provides a new application of the nano-structure hollow calcium carbonate microsphere. According to the microbe microcapsule disclosed by the invention, the nano-structure hollow calcium carbonate microsphere is added in the preparation process of the microbe microcapsule, because a large quantity of pore passage structures exist in the nano-structure hollow calcium carbonate microsphere, the microbes can smoothly enter the nano-structure hollow calcium carbonate microsphere through the pore passages to be coated so as to obtain good protection, and the survival rate of the microbes is far higher than that of a freeze drying process and a spray drying process and basically higher than that of a microencapsulated freeze drying process by carrying out viable counting on the microbe microcapsule. The preparation method disclosed by the invention has the advantages of simple process, small equipment investment, short period and suitability for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of microbial microencapsulation, and in particular relates to the application of nanostructure calcium carbonate hollow microspheres in microbial microencapsulation cultivation. Background technique [0002] Microencapsulation technology is the use of natural or polymer encapsulation materials to encapsulate solid, liquid or gas core substances to form microcapsules with semi-permeable or sealed capsules. The microencapsulation technology of microorganisms, especially the microencapsulation technology of probiotics can solve the survival rate of probiotics very well. At present, there are many studies on the survival rate of probiotics, and the survival rate of microencapsulated freeze-drying is currently the highest, but its disadvantages The equipment investment is large and the production process cycle is long. Contents of the invention [0003] Purpose of the invention: In view of the problems and def...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 张志成胡志华戴鼎震
Owner 靖江市城中村投资建设有限公司
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