microfluidic system
A microfluidic system, fluid technology, applied in fluid controllers, magnetic objects, laboratory containers, etc., can solve problems such as poor separation ability
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example 1
[0229] Example 1: Particle Extraction
[0230] Experiments were performed using two types of devices. Device 1 consisted of a single magnetic tip made of a soft magnetic alloy (AFK502R, Imphy Alloys Arcelor Mittal). An electromagnetic coil (33.5 mm in diameter) made of 1000 turns of copper wire was used to control the magnetization of one tip. The magnetic tip was placed perpendicular to a Teflon tube (300 μm ID and 600 μm OD, Sigma-Aldrich).
[0231] A homemade cylindrical electromagnetic coil consisting of approximately 1000 turns of insulated copper wire (0.8 mm in diameter) was used. The amperage used was from 0A to 4A.
[0232] In a second type of device (device 2), a second tip opposite the first tip can be added to form a magnetic clamp configuration (reflection symmetry).
[0233] exist figure 2 The different steps of the implemented method are shown in .
[0234] Using an automated pipette robotic system, droplet strings are generated in Teflon tubes.
[023...
example 2
[0267] Example 2: Immunoassays within droplets
[0268] As previously described (Example 1), the basic operational units required for immunoassays can be implemented using a droplet platform: bead confinement, bead washing, bead release and mixing within a given droplet, and continuous fluorescence monitoring.
[0269] The immunoassay developed in this example is a sandwich immunoassay in which the capture antibody is grafted onto magnetic particles (from microparticles to nanoparticles). Secondary antibodies (detection antibodies) can be fluorescently labeled (FITC, Alexa…) or conjugated to enzymes (alkaline phosphatase, horseradish peroxidase, etc.). Immunoassays are based on the capture of the analyte of interest by capture antibodies grafted onto beads, while detection is performed using secondary antibodies targeting different epitopes. Analyte quantification is based on the amount of detectable secondary antibody. Using the magnetic droplet platform developed here, i...
example 3
[0297] Example 3: Magnetic bead-based immunoagglutination assay within a confined droplet
[0298] Such as Figure 9 As shown, the agglutination step is carried out within a water-in-oil droplet 30 and is generated by magnetic confinement to enhance the collision frequency of magnetic beads (MB) M, thereby promoting aggregate formation [17].
[0299] Adduction-confined droplets in fluorinated oils30 [18, 19] allow individual compartmentation, preventing cross-contamination. Furthermore, the possibility to generate large quantities of droplets in a "pipeline" format enables reliable and high-throughput analysis with a simple chip design.
[0300] First demonstration of the implementation of this assay using streptavidin-coated MB (1 μm) M (surface-functionalized magnetic particles) and biotinylated alkaline phosphatase (b-PA) (target: 38) as models ( Figure 9 ). As described in detail below, the magnetic particle M provides a plurality of binding sites 39a.
[0301] Dropl...
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