Enteromorpha prolifera feed additive as well as preparation method and applications thereof

A feed additive and enteromorpha technology, applied in the field of feed additives, can solve problems such as complicated methods, low utilization rate of enteromorpha, inconvenient operation, etc.

Active Publication Date: 2014-07-23
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the above methods are complicated and inconvenient to oper

Method used

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  • Enteromorpha prolifera feed additive as well as preparation method and applications thereof
  • Enteromorpha prolifera feed additive as well as preparation method and applications thereof
  • Enteromorpha prolifera feed additive as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the preparation of feed additive

[0041] Enteromorpha collection and cleaning: From March to April 2012, enteromorpha raw materials were collected from various tidal flats in Jimei, Xiang'an, Tong'an, Xiamen Island, and Quanzhou Bay, filtered and rinsed with seawater, mud removed, brought back to the laboratory, and continued to use tap water Rinse 2 times.

[0042] Drying treatment of Enteromorpha: Drain the cleaned Enteromorpha, dry it in the air until there is no water drop left, put it in a 60°C oven and dry it until it can be manually crushed into small pieces;

[0043] Ultrafine pulverization of Enteromorpha: Use a low-temperature pulverizer at a temperature of 10°C to make algae powder from the small flakes of Enteromorpha after drying, and pass through a 60-mesh sieve to obtain Enteromorpha algae powder;

[0044]Anaerobic fermentation: Add 0.1% cellulase (Novozymes compound plant hydrolase ViscozymeL), 0.05% yeast powder (5 billion cfu / g) and 0.0...

Embodiment 2

[0046] Embodiment 2: aquaculture test

[0047] 1) Breeding experiment of Xingzhou red fish

[0048] Add 0%, 1%, 2%, 3%, and 4% to the commercial compound feed for Xingzhou red fish (tilapia compound feed purchased from Xiamen Yinxiang Feed Co., Ltd., see Table 1 and Table 2 for ingredients) , 5% fermented Enteromorpha, mixed evenly, using CD4×17S multifunctional catalytic molding machine to make granular feed with a particle size of 2.5mm, and fed with an average body weight of 4.69±1.98g and a body length of 5.16±0.63cm Sin Chew redfish seedlings. According to the addition amount of fermented Enteromorpha 1%, 2%, 3%, 4%, and 5%, the experiment was divided into 5 groups and a control group (addition amount was 0%), with 3 parallel fish in each group and 30 fish in each tank. The experiment period is 49 days. In the formal test, the feeding amount should be determined according to about 3% of the fish body weight, and adjusted according to the feeding situation. About 50%, ...

Embodiment 3

[0103] Embodiment 3: Feed Additive Orthogonal Test

[0104] Add different amounts of cellulase (Novozymes compound plant hydrolase ViscozymeL), yeast powder (5 billion cfu / g of Saccharomyces cerevisiae) and lactic acid bacteria powder (Lactobacillus acidophilus 3 billion cfu / g) to the enteromorpha algae powder ) (soak the bacterial powder for 4 hours before use) for orthogonal experiments, as shown in Table 13, each group was added with 70% water, mixed evenly, put into a fermentation bag with a specification of 350*450mm and a thickness of 10 silk, and sealed for fermentation 5 days, and determine the relevant indicators. Table 13 is the combination of orthogonal experiments under different conditions.

[0105] Table 13 Orthogonal experiment grouping:

[0106] factor A B C D 1 0 0 0 30 2 0 0.05 0.05 25 3 0 0.10 0.10 35 4 0.05 0 0.05 35 5 0.05 0.05 0.10 30 6 0.05 0.10 0 25 7 0.10 0 0.10 25 8 0.10 0.0...

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Abstract

The invention discloses an enteromorpha prolifera feed additive as well as a preparation method and applications thereof. The preparation method of the enteromorpha prolifera feed additive comprises the following steps: collecting and cleaning enteromorpha prolifera; drying the enteromorpha prolifera; grinding enteromorpha prolifera to obtain enteromorpha prolifera algae powder, then performing anaerobic fermentation, adding cellulase, yeast powder and lactic acid bacterium powder, adding a certain amount of water, evenly mixing, filling in a fermentation bag, and airtighly fermenting to obtain enteromorpha prolifera fermentation liquor, namely the feed additive. The invention further discloses a preparation method of the enteromorpha prolifera feed additive, and applications of the enteromorpha prolifera feed additive for an aquatic feed. The feed additive can maximally utilize the nutritional substances of enteromorpha prolifera, is simple to operate, and convenient to use, and capable of extremely promoting the growth of aquatic products. In addition, the multiple indexes of the aquatic products fed by the enteromorpha prolifera feed additive has good effects.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to an Enteromorpha feed additive, a preparation method and an application thereof. Background technique [0002] In recent years, a series of studies on the application of algae in the aquatic feed industry have been carried out at home and abroad, especially the international research on the application of algae in the aquatic feed industry is more systematic and in-depth. Many countries have carried out commercial production of algae, especially microalgae, while China's research in this area started late, and the research is not systematic. It only made some studies on the types, usage methods, usage amounts, and usage effects of algae that can be used in aquatic feed. Research. [0003] Soler-Vila et al. used different levels (5%, 10% and 15%) of macroalgae ( Porphyra dioica ) powder with equal nitrogen and equal fat compound feed to feed rainbow trout ( Oncorhynchus mykiss )...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00A23K1/18A23K10/12A23K10/30A23K50/80
Inventor 黎中宝杨欢陈强李文静黄永春
Owner JIMEI UNIV
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