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Silk fibroin cartilage complex artificial ear and preparation method thereof

A complex and silk fibroin technology, applied in prosthetics, medical science, etc., to achieve the effect of promoting epithelial crawling, good biocompatibility, and promoting cell growth

Inactive Publication Date: 2014-07-23
EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the application of silk fibroin in auricle reconstruction at home and abroad.

Method used

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  • Silk fibroin cartilage complex artificial ear and preparation method thereof
  • Silk fibroin cartilage complex artificial ear and preparation method thereof
  • Silk fibroin cartilage complex artificial ear and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] 1) Preparation of silk fibroin

[0052] Use waste mulberry silkworm raw silk to degummed in NaHCO3 (0.5wt%) aqueous solution, dissolve the degummed silk in 9.3mol / L LiBr aqueous solution, stir until the silk is completely dissolved, filter, remove undissolved silk and impurities, and obtain silk fibroin Salt solution, remove the LiBr in the solution by dialysis with deionized water, get the silk protein that concentration is 4%, concentrate again, PEG dialysis, the centrifugation of gained silk fibroin solution removes impurity, stay supernatant, the silk fibroin concentration of gained is 10%, store in a 4°C refrigerator for later use;

[0053] 2) Preparation of silk fibroin scaffold

[0054] Mix the prepared silk fibroin solution with n-butanol 1:1, stir for 5 minutes (25°C), put into a 5cm×5cm polystyrene disk, then put it into a -20°C refrigerator for 24 hours, at room temperature, The preparation of the silk fibroin porous scaffold is completed; then the residual...

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Abstract

The invention belongs to the field of tissue engineering and medical materials, and in particular relates to a silk fibroin cartilage complex artificial ear, a preparation method thereof and application thereof in auricle reconstruction. The silk fibroin cartilage complex artificial ear is made by adopting of a silk fibroin cartilage complex as a support material and silk fibroin as a coating. The silk fibroin cartilage complex artificial ear can be further used in the auricle reconstruction of abnormal microtia. The silk fibroin cartilage complex artificial ear has good biocompatibility, promotes the growth of cells, can avoid the injury caused by autologous rib taking, reduces complications, and can be effectively survived for a long term or permanently; the silk fibroin cartilage complex artificial ear solves the rejection problem caused by other biological materials in the auricle reconstruction, also can avoid the injury caused by autologous rib taking, and provides a significant reference for the clinical auricle reconstruction and application.

Description

technical field [0001] The invention belongs to the field of tissue engineering and medical materials, and in particular relates to a silk fibroin cartilage complex artificial ear, a preparation method thereof and an application in auricle reconstruction. Background technique [0002] Studies have shown that congenital microtia is a relatively common head and face deformity. According to statistics, its incidence rate is 0.4-5.5 / 10000. Cartilage and part of the earlobe, even without the above structures, and often accompanied by symptoms such as external auditory canal atresia, middle ear deformity, and maxillofacial deformity. The deformity or lack of auricle seriously affects the patient's appearance and mental health. Faced with the pressure caused by microtia, many patients cannot adapt well to society. Studies have shown that the detection rate of psychological problems in patients with microtia is significantly higher than that of healthy people. Therefore, the first ...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/22
Inventor 倪玉苏江毅文建川陈新邵正中于慧前孙珊李雯李华伟
Owner EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV