Mercapto-containing chitosan derivative, compound nano particle and preparation method

Abstract

The invention discloses a mercapto-containing chitosan derivative, a compound nano particle and a preparation method. The preparation method of the mercapto-containing chitosan derivative includes steps of: (1) preparing a polyethylene glycol-based chitosan; (2) adding a partially-sulfhydrylated polyethylene imine to the polyethylene glycol-based chitosan to mix, adding a catalyst, carrying out a reaction, filtering the mixture, dialyzing with pure water, freeze drying to obtain the mercapto-containing chitosan derivative. The mercapto-containing chitosan derivative contains abundant active groups and can be widely used in the biomedical technical fields of medicament carriers, gene carriers, hybrid materials, etc. A mercapto-containing antineoplastic pro-drug is combined with gold nanorods by Au-S bonds to obtain to obtain a compound nano particle of the mercapto-containing chitosan derivative, wherein the nano particle has dual functions of photothermal therapy and chemotherapy]. The nano particle, with good stability under a high temperature condition or a laser-irradiation condition due to a strong chemical bonding effect, has not only a chemotherapy effect as an antineoplastic drug but also an photothermal-ablation effect on tumours.

Description

technical field [0001] The invention relates to a thiol-containing chitosan derivative and composite nanoparticle and a preparation method thereof. Background technique [0002] Malignant tumor has become the second highest mortality disease after cardiovascular disease in the world, which is a serious threat to human health. Research on cancer treatment has aroused widespread concern. In recent years, nanostructures and nanotechnology have become hotspots in scientific research, and more and more have been applied to the field of biomedicine. Tumor therapy based on nanotechnology has become the latest trend in tumor treatment. [0003] The chitosan molecular structure is rich in active groups such as amino groups and hydroxyl groups, which usually show strong affinity and modifiability. [1] . Use groups with certain characteristics to chemically modify the active hydroxyl and amino groups of chitosan molecular structure, improve the physical and chemical properties and b...

AI Technical Summary

Problems solved by technology

However, the composite nanoparticles in current reports are usually obtained by combining compounds with gold nanorods through electrostatic force, and at the same time binding anti-tumor drugs through physical entrapment, which makes the nanoparticles in the process of tumor treatment due to high temperature or laser Irradiation is prone to reunion or dissociation, affecting the therapeutic effect

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