A rare achromobacter strain and its application in removing heavy metal ions
A rare achromobacter and heavy metal ion technology, applied to microorganisms and its application in the removal of heavy metal ions, rare achromobacter and its application in the removal of heavy metal ions, can solve the problem of large impact, strain screening, bacterial There are few studies on the growth conditions of species characteristics, and there are few heavy metal-tolerant populations.
Inactive Publication Date: 2016-03-16
江苏原元生物工程有限公司
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Problems solved by technology
In the past ten years, the research on heavy metal-resistant microorganisms in my country has mainly focused on the properties of microorganisms and the influence of heavy metals on bacterial cells, while there are few studies on strain screening, strain characteristics, and growth conditions.
At present, there are few isolated heavy metal-resistant populations, the growth and reproduction of bacteria are greatly affected by the habitat environment, and the ability to remove heavy metals is not strong, and it cannot meet the complex pollution status of the external actual environment. ability to remove
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[0111] Removal of heavy metal ions Cd 2+ Strain screening
[0112] 1 Experimental materials
[0113] 1.1 Experimental strains
[0114] Sample source
[0115] Soil samples or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were taken from 1-4 cm of surface soil at multiple locations in the sampling area and mixed separately. There are 27 samples in total.
[0116] 1.2 Experimental equipment
[0117] Vertical pressure steam sterilizer (HirayamaHVE-50, Japan); Air bath shaker (IKAKS4000ic, Germany); Analytical balance (MettlerToledoPL602-S, Switzerland); Constant temperature incubator (MMMIncucell111, Germany); Pipette gun (Eppendorf, Germany); desktop high speed Centrifuge (Eppendorf 5417R, Germany); Inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7700X, USA), etc.
[0118] 1.3 Experimental reagents
[0119] Cadmium Nitrate [Cd(NO 3 ) 2 ×4H 2 O] The chemical reagents are of analytical grade. Calculate the ...
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The present invention discloses a strain of Achromobacter insolitus CC6, and an application of the Achromobacter insolitus CC6 in heavy metal ion removing, wherein the Achromobacter insolitus CC6 provides a heavy metal ion Cd<2+> removing effect. The method for screening Achromobacter insolitus providing a heavy metal ion Cd<2+> removing effect from soil and water comprises: a, preparing a metal selective culture medium; b, carrying out enrichment acclimatization on a heavy metal resistance strain; c, screening and re-screening the heavy metal resistance strain; and d, detecting the heavy metal removing capability of the strain. According to the present invention, with the detection test on the heavy metal removing capability of the strain obtained by carrying out enrichment acclimatization on the heavy metal resistance strain with the metal ion-containing culture medium and purification, the reasonable and effective screening method for the heavy metal removing strain is established so as to obtain the strain suitable for requirements; and with application of the Achromobacter insolitus CC6 in the heavy metal pollution treatment, advantages of no secondary pollution, high treatment efficiency, wide application range, low cost and the like are provided.
Description
Technical field [0001] The invention relates to a strain of microorganism and its application in removing heavy metal ions, in particular to a rare achromobacterium and its application in removing heavy metal ions, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Heavy metals are widely distributed in the atmosphere, biosphere, lithosphere, and hydrosphere. After being discharged into the environment, they are permanent and have obvious cumulative effects. They can pollute water and soil and eventually accumulate in organisms through the food chain cycle. Endanger the ecological environment and human health. [0003] Microbial treatment of heavy metal pollution is an emerging field that is constantly being explored and developed in the world. The use of biosorbents prepared by microbial systems to treat heavy metal pollution is currently the most promising method for heavy metal pollution treatment. Compared with tradit...
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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/025C02F101/20
Inventor 孙军郝莹李凯赵晗
Owner 江苏原元生物工程有限公司