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Liquid microbial agent for microbial degradation of food source organic waste and preparation method thereof

A technology for microbial degradation and organic waste, which is applied in the field of preparation of microbial degradation liquid bacterial agents, can solve problems such as complex processes, and achieve the effect of rich nutrition and quality safety

Active Publication Date: 2016-01-20
北京申城生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the organic waste degrading functional bacteria used in these methods include fungi and bacteria, and the preparation process needs to be fermented and mixed separately, so the process is relatively complicated

Method used

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  • Liquid microbial agent for microbial degradation of food source organic waste and preparation method thereof
  • Liquid microbial agent for microbial degradation of food source organic waste and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the preparation of food source organic waste degrading microbial liquid inoculum, carry out the following steps successively:

[0049] (1) Activation of inclined-plane strains:

[0050] 1. Pick a slant colony of Paenibacillus polymyxa (Paenibacillus polymyxa) F8 with a collection number of CGMCCNO.7087 and inoculate it on a beef extract peptone solid medium plate;

[0051] 2. Pick a slant colony of Bacillus subtilis (Bacillus subtilis) F10 with a collection number of CGMCCNO.7088 and inoculate it on another beef extract peptone solid medium plate;

[0052] ③. Pick a slant colony of Pseudomonas sp. No. 7 with a collection number of CGMCC NO.4133 and inoculate it on another beef extract peptone solid medium plate;

[0053] All the plates above were cultured at 30°C for 2 days to obtain activated colonies of each strain; namely, activated Paenibacillus polymyxa F8, activated Bacillus subtilis F10, activated Pseudomonas (Pseudomonas sp.) No. 7.

[0054] (2)...

Embodiment 2

[0069] Embodiment 2, the preparation of food source organic waste degrading microorganism liquid bacterial agent, carry out the following steps successively:

[0070] Steps (1) and (2) are the same as in Example 1.

[0071] (3), mixed bacterial fermentation culture:

[0072] 1. Prepare the Paenibacillus polymyxa F8 bacterium liquid, the Bacillus subtilis F10 bacterium liquid and the Pseudomonas sp. The volume ratio of 1 is mixed; the mixed bacterial liquid is obtained.

[0073] ②. Preparation of culture medium:

[0074] Add 59 times the volume of water (that is, dilute 60 times) to 1 volume of concentrated monosodium glutamate waste liquid, adjust the pH to 7.3 with alkali, and place it in an empty tank for sterilization (maintain tank pressure 0.2Mpa, tank temperature 130°C, Keep it in a fermenter for 40 minutes); then sterilize it in a solid tank (keep the tank pressure at 0.1Mpa, tank temperature at 120°C, keep it for 30 minutes), cool to room temperature and set aside; ...

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Abstract

The invention discloses a microbial degradation liquid bactericide for food-borne organic refuse. According to the invention, Paenibacillus polymyxa F8, Bacillus subtilis F10 and Pseudomonassp No. 7 are used A preparation method for the bactericide comprises the following steps: 1) activating the three bacteria respectively; 2) performing seed culture on the three activated bacteria respectively; and 3) performing mixed bacteria fermentation culture: mixing the obtained three kinds of bacteria liquid to obtain mixed bacteria liquid; diluting concentrated monosodium glutamate waste liquid, then adjusting the pH to 5.9-8.5 by using alkali, and sterilizing to obtain a culture solution; and inoculating the mixed bacteria liquid to a fermentation tank for fermentation culture according to the inoculation quantity of 5-30% of the volume of the culture solution, thus obtaining the microbial degradation liquid bactericide for the food-borne organic refuse.

Description

technical field [0001] The invention belongs to the field of domestic waste disposal, and relates to a technology for microbial degradation and elimination of food-source organic waste, in particular to a preparation method of a liquid bacterial agent for microbial degradation of food-source organic waste. Background technique [0002] With the rapid development of my country's social economy, not only the amount of household garbage of urban residents is increasing, but also the proportion of food-source organic garbage including melon peels and shells, leftovers and leftovers in household garbage is also increasing. Due to the high moisture content (90%) and low incineration calorific value (2100-3100kJ / kg) of food source organic waste, after being mixed with other urban waste components, landfill disposal will not only occupy valuable land resources but also produce a large amount of leachate And pollute the groundwater system; use incineration to generate electricity for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01C12R1/125
Inventor 方萍李田宇韦杏花
Owner 北京申城生物科技集团有限公司
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