Non-biological method for screening effective antivirus components in traditional Chinese medicines

An active ingredient and anti-virus technology, applied in the field of screening and identification of active compounds, can solve the problems of easy loss of active ingredients, disparity in ingredient content, time-consuming and labor-intensive problems, and achieve the goal of speeding up the process of drug screening, great practical significance, and broad application prospects Effect

Inactive Publication Date: 2014-07-23
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

However, the compounds contained in traditional Chinese medicine and its compound are of various types, which are natural combinatorial chemical libraries, and the contents of each component are very different, and many components are unknown, which leads to time-consuming and laborious separation and purification of active components, and it is easy to lose traces of active components.

Method used

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  • Non-biological method for screening effective antivirus components in traditional Chinese medicines
  • Non-biological method for screening effective antivirus components in traditional Chinese medicines
  • Non-biological method for screening effective antivirus components in traditional Chinese medicines

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with specific embodiments.

[0027] Where the methods used in the following examples are not specifically stated, they are conventional methods.

[0028] 1. Preparation of oseltamivir molecularly imprinted polymer and its liquid chromatography column

[0029] The parameters in the polymer preparation process were optimized by uniform design method, oseltamivir (template molecule) 0.1 mmol, functional monomer 0.69 mmol, ethylene glycol dimethacrylate (crosslinking agent) 5 mmol, 30% acrylamide and 70% 4-vinylpyridine as a functional monomer, toluene as a porogen, and 15 mg of azobisisobutyronitrile as an initiator, and a 60°C oil bath heat-initiated the polymerization reaction to obtain a hard white block polymer. Grinding and sieving to collect particles with a particle size between 25 and 45 μm, and eluting them with methanol-acetic acid (90:10, V / V) in a Soxhlet extractor to remove templat...

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Abstract

The invention discloses a non-biological method for screening effective antivirus components in traditional Chinese medicines. The method comprises the following steps: taking an influenza virus specific drug oseltamivir (OS) as the template molecule, taking acrylamide and 4-vinylpyridine as the functional monomers, carrying out synthesis to obtain non-covalent oseltamivir molecular imprinting polymer (OS-MIP), taking the OS-MIP as the stationary phase to prepare a liquid chromatographic column through a wet method, carrying out affinity screening on effective component groups of traditional Chinese herbals and compounds, identifying the compound components with affinity through mass spectrum, then carrying out in-vitro anti-virus experiments to verify the compound activity, and finally finding the compounds, which have the same antivirus activity as the template molecule, in the traditional Chinese medicines. The method can also be modified to screen and verify other active compounds so as to shorten the screening process of drug entities and promote the research process of replacement drugs, and has a very important meaning and wide application prospect in the field of non-biological drug screening methods.

Description

technical field [0001] The invention relates to a method for screening and identifying active compounds, in particular to a non-biological method for separating, screening and identifying compounds with activities similar to predetermined template molecules from traditional Chinese medicines. [0002] Background technique [0003] The research and development of new drugs based on chemical synthesis is becoming more and more difficult, the funding investment is increasing exponentially, and the research and development cycle is getting longer and longer. Therefore, more and more new technologies are used in the screening and development of drugs, such as combinatorial chemistry methods: rapid production and establishment of libraries containing thousands of compounds; high-throughput drug screening technology: using various fast and efficient screening and detection methods To discover drug precursors; genomics, proteomics, metabolomics, ADMETox: advance the study of pharma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14
Inventor 李剑勇杨亚军刘希望张继瑜李冰周绪正刘玉荣魏小娟牛建荣
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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