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Method of extracting, purifying and inhibiting alpha-glucosaccharase active ingredient from chestnut shell

A technology of glucosidase, active ingredient, applied in the field of natural product chemistry

Inactive Publication Date: 2014-07-30
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A variety of traditional Chinese medicine extracts also show significant inhibitory effects on α-glucosidase. Studies have pointed out that flavonoids, alkaloids, terpenoids, phenols and other substances can effectively inhibit the activity of α-glucosidase, but No reports have been found so far that substances with inhibitory α-glucosidase activity have been isolated from chestnut shells

Method used

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  • Method of extracting, purifying and inhibiting alpha-glucosaccharase active ingredient from chestnut shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) 100g of chestnut shells were washed, dried and crushed, and 75% (v / v) ethanol was added according to the ratio of material to liquid 1:10 (w / v) and boiled and refluxed twice, each time for 2 hours;

[0017] (2) Combine the chestnut shell extracts and concentrate under reduced pressure at 50°C for 2 hours to obtain a concentrated extract;

[0018] (3) The concentrated extract was prepared into a 10 mg / mL solution with water, and then extracted 3 times with 4 times the volume of water-saturated n-butanol, each time for 2 hours;

[0019] (4) Combine the water-saturated n-butanol extracts, concentrate under reduced pressure at 70°C for 2 hours to obtain a concentrated extract, and then freeze-dry at -70°C for 10 hours to obtain 0.5g of powdery extract;

[0020] (5) The powdery extract was made into a 0.5 mg / mL solution with water, adsorbed with macroporous resin AB-8, eluted with 60% (v / v) ethanol at a flow rate of 1 BV / h, and collected the eluate;

[0021] (6) measure...

Embodiment 2

[0023] (1) 10kg of chestnut shells were washed, dried and crushed, and 75% (v / v) ethanol was added according to the ratio of material to liquid 1:10 (w / v) and boiled and refluxed for 3 times, each time for 4 hours;

[0024] (2) Combine the chestnut shell extracts and concentrate under reduced pressure at 50°C for 4 hours to obtain a concentrated extract;

[0025] (3) The concentrated extract was prepared into a 10 mg / mL solution with water, and then extracted 5 times with 4 times the volume of water-saturated n-butanol, each time for 4 hours;

[0026] (4) Combine the water-saturated n-butanol extracts, concentrate under reduced pressure at 80°C for 4 hours to obtain a concentrated extract, and freeze-dry at -90°C for 24 hours to obtain 50 g of powdery extract;

[0027] (5) The powdery extract was made into a 0.5 mg / mL solution with water, adsorbed with macroporous resin AB-8, eluted with 60% (v / v) ethanol at a flow rate of 1 BV / h, and collected the eluate;

[0028] (6) Measur...

Embodiment 3

[0030] (1) 100kg of chestnut shells were washed, dried and crushed, and 75% (v / v) ethanol was added according to the ratio of material to liquid 1:10 (w / v) and boiled and refluxed twice, each time for 3 hours;

[0031] (2) Combine the chestnut shell extracts and concentrate under reduced pressure at 50°C for 3 hours to obtain a concentrated extract;

[0032] (3) The concentrated extract was prepared into a 10 mg / mL solution with water, and then extracted 4 times with 4 times the volume of water-saturated n-butanol, each time for 3 hours;

[0033] (4) Combine the water-saturated n-butanol extracts, concentrate under reduced pressure at 75°C for 3 hours to obtain a concentrated extract, and then freeze-dry at -80°C for 12 hours to obtain 450g of powdery extract;

[0034] (5) The powdery extract was made into a 0.5 mg / mL solution with water, adsorbed with macroporous resin AB-8, eluted with 60% (v / v) ethanol at a flow rate of 1 BV / h, and collected the eluate;

[0035] (6) Measur...

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Abstract

The invention relates to a method of extracting, purifying and inhibiting an alpha-glucosaccharase active ingredient from a chestnut shell. The method comprises the following steps: firstly, cleaning the chestnut shell, drying, crushing, refluxing and extracting by 75% (v / v) alcohol, concentrating under reduced pressure, adding water and statured n-butyl alcohol liquor to extract; and finally, carrying out adsorption elution by using macroporous resin AB-8 to obtain polyphenol extract with effect of inhibiting activity of alpha-glucosaccharase.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and particularly relates to a method for extracting and purifying active ingredients inhibiting alpha-glucosidase from chestnut shells. Background technique [0002] The chestnut shell is the shell of the Fagaceae plant chestnut (Castanea mollissima Blume). It is a deciduous tree, generally 15 to 20 meters high, suitable for planting on the sandy loam soil on the sunny slopes of the mountains, and is widely cultivated in the north and south of my country. The fruit is spherical in shape, with needles, and the spines are densely covered with soft hairs. When ripe, they crack and release the nuts. [0003] The pharmacological effects of chestnut shell mainly include antibacterial, anti-inflammatory, anticoagulant, treatment of chronic bronchitis, and treatment of bacillary dysentery. Now the research on chestnut shell mainly focuses on the extraction of pigments and biologically active subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/04A61P3/10
Inventor 欧阳杰刘莉唐新玥张欣珂王珺璟
Owner BEIJING FORESTRY UNIVERSITY
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