Aqueous two-phase method for efficiently extracting phycoerythrin
A phycoerythrin, two-phase technology, which is applied in the preparation method of peptides, algae/moss peptides, chemical instruments and methods, etc., achieves the effects of fast mass transfer, low cost and mild separation conditions
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[0019] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0020] figure 1 Four different salts are compared in : sodium phosphate, potassium phosphate, potassium sodium tartrate, ammonium sulfate. In the comparison of different PEG molecular weights, potassium sodium tartrate has the highest purity.
[0021] figure 2 Four different PEG molecular weights were compared: 1000, 1500, 2000, 4000. PEG2000 has the highest purity.
[0022] image 3 Four different tether lengths and different volume ratios were compared: the tether length was 22.5%, and the purity of the volume ratio was 0.33.
[0023] Obtain optimal parameters and establish an efficient two-phase aqueous system. Extract phycoerythrin. The characteristics of simple operation, short extraction time, no selectivity and no secondary pollution make it possible to become a common method for protein separa...
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