Aqueous two-phase method for efficiently extracting phycoerythrin

A phycoerythrin, two-phase technology, which is applied in the preparation method of peptides, algae/moss peptides, chemical instruments and methods, etc., achieves the effects of fast mass transfer, low cost and mild separation conditions

Inactive Publication Date: 2014-07-30
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Select PEG / salt / water system, extract phycoerythrin multiple times, and meet the standards of all levels, no report

Method used

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  • Aqueous two-phase method for efficiently extracting phycoerythrin
  • Aqueous two-phase method for efficiently extracting phycoerythrin
  • Aqueous two-phase method for efficiently extracting phycoerythrin

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Embodiment Construction

[0019] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0020] figure 1 Four different salts are compared in : sodium phosphate, potassium phosphate, potassium sodium tartrate, ammonium sulfate. In the comparison of different PEG molecular weights, potassium sodium tartrate has the highest purity.

[0021] figure 2 Four different PEG molecular weights were compared: 1000, 1500, 2000, 4000. PEG2000 has the highest purity.

[0022] image 3 Four different tether lengths and different volume ratios were compared: the tether length was 22.5%, and the purity of the volume ratio was 0.33.

[0023] Obtain optimal parameters and establish an efficient two-phase aqueous system. Extract phycoerythrin. The characteristics of simple operation, short extraction time, no selectivity and no secondary pollution make it possible to become a common method for protein separa...

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Abstract

The invention discloses an aqueous two-phase method for efficiently extracting phycoerythrin. The aqueous two-phase method comprises the following steps: (1) drawing an aqueous two-phase diagram; (2) selecting two-phase points, and carrying out a preliminary experiment; (3) comparing the extraction purity of different salt systems, and selecting an appropriate PEG (polyethylene glycol)/salt system; (4) comparing the influence of different parameters such as different molecular masses of PEG, different system line lengths, different volume ratios and different pH values on purity; and (5) carrying out multiple aqueous two-phase experiments, and selecting the optimal number of times. The aqueous two-phase method simplifies a protein purification process, is easy to operate on a large scale, low in cost, high in yield, high-efficiency and environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of protein extraction and purification, and relates to a two-phase aqueous method for efficiently extracting phycoerythrin. Background technique [0002] Phycoerythrin is mainly obtained through extraction from algae, including algae species selection and large-scale selection, cultivation, collection of algae, crushing algae cells and bioseparation of proteins. Moreover, the complexity of the protein purification process, high cost, and low yield lead to extremely high market prices for natural phycobiliproteins, as high as 50 to 150 US dollars per mg of phycobiliproteins. At present, the production of phycobiliproteins by genetic engineering technology generally requires rough extraction of phycobiliproteins, and then multi-stage chromatography column chromatography. Column chromatography methods based on different separation principles mainly include adsorption chromatography and ion exchange chromatograp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/14
CPCC07K14/405
Inventor 赵丽蔡伟民彭一良
Owner HARBIN INST OF TECH
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