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Closely-linked molecular marker of new gene Xa39 resistant to rice xanthomonas oryzae

A bacterial blight resistance and molecular marker technology is applied in the field of rice disease resistance breeding and molecular genetics, and can solve the problems of narrow resistance spectrum, limited resistance genes, and difficulty in transfection of disease resistance genes at the adult stage. To achieve the effect of speeding up the breeding process

Active Publication Date: 2014-07-30
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

So far, 38 bacterial blight resistance genes have been identified at home and abroad. However, due to the difficulty of transgenic resistance genes from wild rice, some resistance genes only have adult plant resistance, and most of the resistance genes have relatively low resistance spectrum. Due to narrowness and other reasons, the resistance genes used in breeding are very limited

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  • Closely-linked molecular marker of new gene Xa39 resistant to rice xanthomonas oryzae
  • Closely-linked molecular marker of new gene Xa39 resistant to rice xanthomonas oryzae
  • Closely-linked molecular marker of new gene Xa39 resistant to rice xanthomonas oryzae

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified.

[0017] (1) Fine mapping of new rice bacterial blight resistance genes and acquisition of closely linked markers

[0018] Through the SSR primers published on the Gramene website, and the sequence differences between the japonica rice variety and the indica rice variety 9311 sequenced on both sides of Xa39 linked markers RM21 and RM206 based on bioinformatics alignment, Indel primers were further designed to amplify FF329 and its parent Huang Huazhan , screened to obtain polymorphic molecular markers.

[0019] F 1 Seed, in F 1 The representative strain PXO99 of bacterial blight was artificially inoculated at the seedling stage of the plants, and a single disease-resistant plant was selected to harvest to obtain F 2 group. For the obtained F 2 Individuals were genotyped in F of 1454...

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Abstract

The invention relates to a molecular marking method of new gene Xa39 resistant to rice xanthomonas oryzae, and belongs to the field of rice disease resistance breeding and molecular genetics. The method essentially comprises the following steps: back-crossing Xa39-carrying xanthomonas oryzae resistant rice to an F2 group of introgression line FF329 and parental Huanghuazhan (a rice variety) according to the linkage separation rules; then performing linkage analysis for the target gene in light of the phenotype of each individual after inoculating xanthomonas oryzae; finely mapping Xa39 to be within the interval of 97Kb in the eleventh chromosome to obtain the closely-linked PCR-based molecular marker RM26985. The molecular marker can be applied to rice breeding for disease resistance to quickly and accurately identify the individual of genotype resistant to xanthomonas oryzae, so as to speed up the rice molecule breeding for disease resistance.

Description

technical field [0001] The invention relates to a closely linked molecular marker of a new rice bacterial blight resistance gene Xa39, which belongs to the field of rice disease resistance breeding and molecular genetics, and is suitable for introducing the bacterial blight resistance gene Xa39 in rice disease resistance breeding. Tightly linked molecular markers were used for assisted selection breeding of rice bacterial blight resistance traits. Background technique [0002] Rice bacterial blight caused by Xanthomonas oryzae pv.oryzae (Xoo) is an important bacterial disease of rice. Disaster is one of the important limiting factors for high and stable rice yield. There is a specific interaction between rice and bacterial blight. Breeding and planting disease-resistant varieties is the most economical and effective way to control rice bacterial blight. So far, 38 bacterial blight resistance genes have been identified at home and abroad. However, due to the difficulty of t...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 周永力张帆黎志康徐建龙卓大龙黄立钰
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI