Preparing method of artificial seeds of hemarthria compressa

A technology of verbenia flat tachycarpus and artificial seeds, which is applied in the field of making artificial seeds of verbena tachycarpus, can solve the problems of unsatisfactory ecological environment construction and low germination rate of artificial seeds, and achieve easy acquisition, fast propagation speed, The effect of high survival rate

Inactive Publication Date: 2014-08-06
SICHUAN AGRI UNIV
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Problems solved by technology

So far, there have been few reports on the vegetative propagation technology of Verbena scutellaria, most of which are propagated by the general method of vegetative propagation, and the germination rate of the artificial seeds of Verbena scutellaria is low, which cannot meet the requirements of ecological environment construction. need

Method used

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  • Preparing method of artificial seeds of hemarthria compressa
  • Preparing method of artificial seeds of hemarthria compressa
  • Preparing method of artificial seeds of hemarthria compressa

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Experimental program
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Effect test

Embodiment 1

[0032] In this embodiment, the solvent of the artificial endosperm in the semi-gel state is MS medium solution, the mass volume ratio of the activated carbon is 0.5%, and the mass volume ratio of sodium alginate is 2%, 3%, 4%, A completely randomized experimental design was adopted, with a total of 3 treatments, and 20 artificial seeds were produced in each treatment group, and the 20 artificial seeds prepared in each treatment group were placed in a petri dish covered with wet filter paper, placed in a place where the temperature could be controlled manually. Cultivate in a light culture room with environmental conditions such as light intensity. The light intensity is 2000lx, 12h / d, and the temperature is 25°C. After 15 days from the date of sowing, the artificial seed characteristics and germination rate are counted, as shown in Table 1:

[0033] Table 1

[0034]

[0035] Note: The statistical method of the germination rate: the artificial seeds were placed in a petri di...

Embodiment 2

[0039] In this embodiment, the solvent of the artificial endosperm in the semi-gel state is MS medium solution, the mass volume ratio of the activated carbon is 0.5%, the mass volume ratio of sodium alginate is 3%, and the content of NAA is 0, 0.1mg / L, 0.2mg / L, 0.3mg / L, hormone GA 3 The content of cytokinin 6-BA was 0, 0.01mg / L, 0.05mg / L, 0.1mg / L, and the content of cytokinin 6-BA was 0, 1mg / L, 2mg / L, 3mg / L. A completely randomized experimental design was adopted. A total of 16 treatments, 20 artificial seeds were made for each treatment group, and the 20 artificial seeds made by each treatment group were placed in a petri dish covered with wet filter paper, placed in a room where the temperature, light intensity and other environmental conditions could be controlled manually. Cultivate in the light culture room, light intensity is 2000lx, 12h / d, temperature 25 ℃, since the date of sowing 15d after statistics artificial seed characteristics and germination rate, as shown in Ta...

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Abstract

The invention discloses a preparing method of artificial seeds of hemarthria compressa capable of increasing the rate of the emergence of artificial seeds. According to the preparing method, stipes of the hemarthria compressa is adopted. The preparing method comprises the following steps: firstly cleaning up the stipes and carrying out disinfection treatment, then preparing artificial endosperm in a semigel state, putting the stipes of the hemarthria compressa in the artificial endosperm, then absorbing a mixture of the artificial endosperm and the stipes by adopting a dropper, wherein one stipes is absorbed every time, then dropping the absorbed mixture into a CaCl2 solution for ion exchange treatment, forming shells on the surface of the stipes to form capsules after a certain time, taking out the capsules and rinsing the capsules by adopting sterile deionized water to obtain the artificial seeds of the hemarthria compressa. The artificial seeds of the hemarthria compressa prepared by the preparing method are high in rate of the emergence, high in breeding speed and high in survival rate. Preparing materials are easy to obtain, the entire preparing process is simple, the seasonal effect is low, and the cost is low. The preparing method is suitable for popularization and application in plant artificial seed production technologies.

Description

technical field [0001] The invention belongs to the technical field of plant artificial seed production, and in particular relates to a method for preparing artificial seed of verbena scutellaria. Background technique [0002] Seeds are the sexual reproductive organs unique to all seed plants. The structure of the seed is generally composed of three parts: embryo, endosperm and seed coat. In terms of source, normal plant seeds in nature are divided into two categories: one is that the plant develops from the ovule after fertilization; the other is that the plant develops directly from the adventitious embryo without fertilization. However, with the development of production and the improvement of people's living standards, only relying on seeds or asexual reproductive organs with limited annual production capacity for production and reproduction cannot meet the demand. Therefore, it is imperative to find an alternative method that can produce and reproduce every year, so w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/06
Inventor 杨春华刘刚陈菲李达旭陈灵鸷刘琳张建孙飞达
Owner SICHUAN AGRI UNIV
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