Bitter herbs polysaccharide extraction method

An extraction method and technology of bitter herbs, applied in the biological field, can solve the problems of unreasonable selection of technical parameters, low polysaccharide content and purity, and achieve the effects of shortening the extraction time, stabilizing the process, and increasing the yield

Inactive Publication Date: 2014-08-06
陈朋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the extraction techniques of bitter vegetable polysaccharide mainly include water extraction centrifugation method and water extraction alcohol precipitation method. However, due to the unreasonable selection of technical parameters in the existing extraction methods, the content and purity of polysaccharides in the extract are not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 100g of dried bitter herbs and grind them into 400 meshes;

[0023] (2) Mix crushed bitter herbs and deionized water, and extract by ultrasonic, among which: bitter herbs: deionized water = 1:4, ultrasonic temperature is 50°C, ultrasonic time is 20min, working for 3 seconds, stopping for 5 seconds, ultrasonic power 800W; centrifuge at 5000rpm to collect the supernatant, repeat the precipitation once according to the above ultrasonic extraction conditions, collect the supernatant by centrifugation at 5000rpm, and combine the supernatant obtained twice to be the extract of bitter cabbage;

[0024] (3) Regulating the vacuum tightness is 0.08Mpa, utilizes decompression concentration to concentrate the extract obtained in step (2) to 1 / 3 of the original volume;

[0025] (4) Add three times the volume of absolute ethanol to the concentrated solution obtained in step (3), let stand for 2 h, and centrifuge to collect the precipitate;

[0026] (5) The precipitate obtai...

Embodiment 2

[0029] (1) Take 100g dried bitter herbs and grind them into 300 meshes;

[0030] (2) Mix crushed bitter herbs and deionized water, and extract by ultrasonic, wherein bitter herbs: deionized water=1:6, ultrasonic temperature is 60°C, ultrasonic time is 30min, work for 3 seconds, stop for 5 seconds, and ultrasonic power is 10000W centrifuge at 5000rpm to collect the supernatant, repeat the precipitation once according to the above-mentioned ultrasonic extraction conditions, collect the supernatant by centrifugation at 5000rpm, and combine the two supernatants to be the extract of bitter cabbage;

[0031] (3) Regulating the vacuum tightness is 0.08Mpa, utilizes decompression concentration to concentrate the extract obtained in step (2) to 1 / 3 of the original volume;

[0032] (4) Add three times the volume of absolute ethanol to the concentrated solution obtained in step (3), let stand for 3 h, and centrifuge to collect the precipitate;

[0033] (5) Dissolve the precipitate obtai...

Embodiment 3

[0036] (1) Take 100g of dried bitter herbs and grind them into 200 meshes;

[0037] (2) Mix crushed bitter herbs and deionized water, and extract by ultrasonic, wherein bitter herbs: deionized water=1:8, ultrasonic temperature is 70°C, ultrasonic time is 40min, work for 3 seconds, stop for 5 seconds, and ultrasonic power is 1200W centrifuge at 5000rpm to collect the supernatant, repeat the precipitation once according to the above-mentioned ultrasonic extraction conditions, collect the supernatant by centrifugation at 5000rpm, and combine the two supernatants to be the extract of bitter cabbage;

[0038] (3) Regulating the vacuum tightness is 0.08Mpa, utilizes decompression concentration to concentrate the extract obtained in step (2) to 1 / 3 of the original volume;

[0039] (4) Add three times the volume of absolute ethanol to the concentrated solution obtained in step (3), let stand for 4 h, and centrifuge to collect the precipitate;

[0040] (5) The precipitate obtained in ...

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PUM

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Abstract

The invention relates to a bitter herbs polysaccharide extraction method. The method comprises the steps of dry crushing, ultrasonic extraction, reduced pressure concentration, ethanol precipitation, dialysis and vacuum drying. The extraction method has the advantages of simplicity, low cost and stable process. The bitter herbs polysaccharide extraction method improves the content of bitter herbs polysaccharide to 0.53% and the purity of the bitter herbs polysaccharide to 82% by optimizing the technological parameters, such as crushing particle size, ultrasonic extraction parameters and semi-permeable membrane permeation size.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting bitter vegetable polysaccharides. Background technique [0002] Bitter cabbage, scientific name Patrinia, is a plant of Patrinaceae, distributed in northern, eastern and southern my country, and is a traditional Chinese medicine and wild vegetable in China. "Shen Nong's Materia Medica" is listed as the top grade, and it is a commonly used heat-clearing and detoxifying medicine. Its nature and flavor are pungent, bitter, slightly cold, and have the functions of clearing away heat and detoxification, eliminating carbuncle and draining pus, removing blood stasis and relieving pain. It is used to treat intestinal carbuncle (appendicitis), pulmonary carbuncle (lung abscess), carbuncle sore and postpartum stasis and abdominal pain. [0003] In recent years, through clinical verification, the substance has many new functions: treatment of neurasthenia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCG06F3/0236G06F3/0489
Inventor 陈朋苏峰
Owner 陈朋
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