Screening method and application of multiple-allele PCR (Polymerase Chain Reaction) amplified fragment

Abstract

The invention discloses a screening method and application of a multiple-allele PCR (Polymerase Chain Reaction) amplified fragment. The screening method comprises the steps of finding the published chromosome related data through NCBI (National Center for Biotechnology Information), and determining SNP (Single Nucleotide Polymorphism) aggregation suspected to exist; then, designing a primer for an SNP aggregation region suspected to exist, and carrying out PCR (Polymerase Chain Reaction); sequencing to detect whether a plurality of SNP sites really exist on a PCR amplified fragment; further carrying out correlation analysis on the SNP sites to determine whether the amplified fragment has multiple alleles so as to screen the multiple-allele PCR amplified fragment for animal individual identification, meat product tracing technology research and industrialization and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a screening method and application of multi-allelic PCR amplification fragments. Background technique [0002] In recent years, food safety incidents have occurred frequently, and government management departments and consumers have paid unprecedented attention to food safety. Therefore, it is imminent to develop animal individual identification technology and meat product traceability technology, especially DNA identification technology. [0003] To this end, we have carried out a series of research on SNP barcode technology for animal individual identification and meat product traceability, and we deeply feel that if we want to truly realize the research and development and real industrialization of animal individual identification and other technologies, we need to make individual animal identification DNA identification and meat products Product DNA traceability technology and ...

AI Technical Summary

Problems solved by technology

Screening for gene interval sequences is not only easy to find a large number of SNP sites, but also the multi-allelic PCR amplification fragments composed of a large number of SNP sites have a large amount of information, which in turn enables individual animal identity DNA identification and meat product traceability. And its research and development has become simpler, and the cost of technology industrialization is lower; compared with the intergenic sequence on the chromosome, the functional gene sequence is relatively conservative, with relatively few mutations, and the SNP screening efficiency is relatively low, and the amplified PCR fragment Most of them contain only one SNP site, and the amount of information is relatively limited

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