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Application of pfp Gene in Controlling Rice Seedling Heat Tolerance

A technology for rice seedlings and heat resistance, applied in the directions of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of improving heat resistance at the seedling stage

Inactive Publication Date: 2016-02-10
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

However, there are few research reports on the cloning of rice heat tolerance genes and their application in rice breeding

Method used

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  • Application of pfp Gene in Controlling Rice Seedling Heat Tolerance
  • Application of pfp Gene in Controlling Rice Seedling Heat Tolerance
  • Application of pfp Gene in Controlling Rice Seedling Heat Tolerance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Experimental materials

[0018] Wild rice heat-sensitive introgression line YIL106 (PFP ​​genotype is the same as wild rice), heat-resistant recurrent parent Teqing, and 77 local rice varieties. Various restriction enzymes used in the experiment, T 4 DNA ligase, TaqPlus enzyme, etc. were purchased from TAKARA Company, PCRBuffer, dNTP, TRIzolRNA extraction kit used in PCR, Escherichia coli (E.coli) TOP10 were purchased from Tiangen Bioengineering Company, reverse transcriptase was purchased from Liuhetong Company The kits used in the gene chip experiment were purchased from Qiagen, the various primers used in the experiment were synthesized by Beijing Aoke Biotechnology Company, and various sequence analyzes were performed by Beijing Liuhe Huada Gene Technology Company.

[0019] 2. PFP gene sequence and heat resistance correlation analysis

[0020] Amplification and sequencing analysis of PFP gene sequence

[0021] In order to obtain the full-length coding sequence...

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Abstract

The invention relates to application of a PFP (Peroxidase Family Protein) gene capable of improving the seedling-stage heat resistance of rice in the genetic improvement of the seedling-stage heat resistance of the rice. According to the application, an overexpression vector of the gene is constructed through cloning the rice seedling-stage heat resistance related gene PFP, transgenic positive plants are obtained by a gene gun method, and the seedling-stage heat resistance of the transgenic plants is improved as compared with that of control; the coding gene disclosed by the invention has important theoretical and practical significance for the improvement of the heat resistance and related traits of the rice; shown by analysis on the relativity between the heat resistant phenotypes of a plurality of typical local rice varieties and the genotype of the heat resistant gene PFP, remarkable relativity exists between the genotype of the gene and the heat resistant phenotypes of the varieties; shown by further research, the heat resistant gene PFP has gene expression increased under the stress of salt and low temperature and has gene expression decreased under the stress of PEG drought; a foundation for deep analysis on the function and expression of the gene is laid, and a support is provided for clarifying a genetic regulation and control network of rice heat resistance.

Description

technical field [0001] The invention relates to the technical field of rice genetic engineering, in particular to the application of a gene PFP related to rice seedling heat resistance obtained through cloning and functional verification in the genetic improvement of rice seedling heat resistance. Background technique [0002] During the growth of plants, they are often subjected to various stresses such as high temperature and drought, which greatly reduce their yield and limit their planting area. With the intensification of global environmental changes and population growth, the pressure on the demand for food is increasing, and there is an urgent need to cultivate crops that grow under various adversities. With the development of molecular biology technology, the research on the mechanism of plant adaptation to adversity has moved from the physiological level to the molecular level. Genetic engineering stress resistance breeding for crops. [0003] Rice is one of the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/08C12N15/82A01H5/00
Inventor 雷东阳
Owner HUNAN AGRICULTURAL UNIV
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