In-vitro culture medium of mycoplasma ovipneumoniae and preparation method thereof

A technology for preparing Mycoplasma pneumoniae and culture medium, applied in the field of bacterial culture medium and preparation thereof, can solve the problems of high cost, complicated configuration, cumbersome process and the like, and achieve the effect of low cost

Inactive Publication Date: 2014-08-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to basic nutrition, the artificial medium used at this stage also needs ingredients such as beef heart infusion, yeast liquid, coenzyme I, amino acids, etc. In addition, 10% to 20% of animal serum and other ingredients need to be added. Its con

Method used

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Examples

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Embodiment Construction

[0009] The following is an embodiment of the present invention.

[0010] Prepare 1000mL culture medium for Mycoplasma ovis pneumoniae: first measure 500mL double distilled water, then weigh tryptone 15g / L, soybean peptone 5g / L, sodium chloride 5g / L, add 10.5g lactose, yeast extract 25% 100mL , 9 mL of 4.5% phenol red, dilute to 900 mL, autoclave at 121°C for 20 minutes, after high pressure, take out and cool to below 37°C, add 100 mL of sterile inactivated healthy pig serum, add sterilized water Dissolve 200,000 U / L of penicillin, adjust the pH value to 7.5 with 1M NaOH, and shake well. Store in a 4°C refrigerator for later use.

[0011] Get this substratum and KM2 substratum of same volume and carry out titer culture, the result shows, substratum of the present invention just changes in 24 hours color, and KM2 substratum needs 72 hours color and just changes, and substratum of the present invention just changes after inoculation. 5 days growth titer reaches 10 -10 ccu, the...

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Abstract

The invention discloses an in-vitro culture medium of mycoplasma ovipneumoniae and a preparation method thereof. The preparation method comprises the steps of mixing tryptone, soy peptone, sodium chloride, lactose, a yeast leaching agent and phenol red, then sterilizing under high pressure, cooling the mixture to below 37 DEG C, then adding 100-200mL/L of deactivated healthy porcine serum and 200 thousand U/L of penicillin dissolved by sterilizing water into the mixture, and regulating the pH value to be 7.4-7.8 with NaOH, so as to obtain the culture medium. Being cultivated by the culture medium, the mycoplasma ovipneumoniae can grow only in 24 hours, the growing titer can reach 10<-10>ccu, and compared with the prior art, the culture medium has simpler composition and lower cost.

Description

technical field [0001] The invention relates to a bacterial culture medium and a preparation method thereof. Specifically, the invention relates to a culture medium for Mycoplasma pneumoniae in vitro and a preparation method for the culture medium. Background technique [0002] Mycoplasma ovis pneumoniae ( Mycoplasma ovipneumoniae , Mo) is a prokaryotic microorganism without a cell wall structure between bacteria and viruses, mainly causing proliferative interstitial pneumonia in sheep, goats, especially lambs. The pathogen was first isolated from the lungs of sheep with lung adenocarcinoma by Mackay et al. (1963), and subsequently Carmichael (1972) named it Mycoplasma ovis pneumoniae. Since then, the pathogen has been reported in many countries and regions, such as New Zealand, Hungary, Iceland, the United Kingdom, Australia, Spain, Turkey, many countries in Africa and West Asia (Jonas et al, 1991; Linvingston et al, 1979), The development of the sheep industry has cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
Inventor 逯忠新贺英赵萍储岳峰陈胜利张建军
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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