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Test strip for specific pest allergen ige screening and preparation method thereof

A technology for test strips and allergens, which is used in measuring devices, instruments, disease diagnosis and other directions, can solve the problems of long test time, poor sensitivity and precision, and cumbersome operation, and achieves simple operation, accurate results and low cost. Effect

Active Publication Date: 2016-03-23
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Comparison scheme
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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example The test strip used for the screening of specific pest allergen IgE and its preparation method

[0029] The preparation of test strips for IgE screening of specific cockroach allergens includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold labeled cockroach antigen, the purification of the colloidal gold cockroach antigen, and the purification of the colloidal gold protein particles. Particle size identification and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[003...

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Abstract

The invention provides a test strip for screening specific injurious insect allergens IgE (Immunoglobulin E). The test strip is coated with a pest antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific injurious insect allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for IgE screening of specific pest allergens and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/532
CPCG01N33/531G01N33/532G01N33/558G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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