Acellular corneal stroma and preparing method for acellular corneal stroma

A corneal stroma and decellularization technology, applied in the field of medical devices, can solve the problems of material contamination, violent reaction, corneal stroma damage, etc., and achieve the effect of avoiding pollution and simple operation

Active Publication Date: 2014-08-27
SHENZHEN AINEAR CORNEA ENG
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Problems solved by technology

[0003] However, in the decellularization process of the acellular corneal stroma produced by the prior art, solutions such as trypsin, EDTA (ethylenediaminetetraacetic acid) and Triton-X100 (polyethylene glycol octylphenyl ether), deoxycholorin Sodium dodecyl sulfate, SDS (sodium dodecylsulfonate) and other surfactants, although these methods can remove cell components, but the reaction is severe, the corneal stroma is severely damaged, and

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[0027] It should be understood that the specific implementations described here are the best implementations for achieving the purpose of the present invention, which are only used to explain the present invention and not to limit the present invention.

[0028] The present invention provides a method for preparing acellular corneal stroma. In the first embodiment, the method for preparing acellular corneal stroma includes the following steps:

[0029] A. Put the removed animal cornea into a reagent bottle filled with water, and then oscillate it in a constant temperature oscillator at 200 rpm (revolutions per minute) for 5 hours (hours), during which time the water is changed every 1 hour To make the epithelial layer fall off. The animals include pigs, cattle, sheep and other animals, and the water used includes injection water, pure water, physiological saline and the like. During the shaking process, the collagen of the corneal stroma absorbs water and becomes fluffy, the corne...

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Abstract

The invention discloses an acellular corneal stroma and a preparing method for the acellular corneal stroma. The preparing method for the acellular corneal stroma includes the following steps: (1) placing a picked animal cornea into water, and carrying out vibration processing to enable the epithelial layer to fall off; (2) replacing the water with acellular reagents, and carrying out vibration processing, wherein during the period, the acellular reagents and the water are alternately used to enable stroma cells and endothelial cells to fall off; (3) adding dehydrating agents, and enabling the mixture to stand or carrying out vibration processing to obtain the acellular cornea; (4) cutting the acellular cornea to obtain the acellular corneal stroma, wherein the acellular corneal stroma only comprises a front elastic layer and a stroma layer. The acellular reagents contain NaCl and EDTA, and the dehydrating agents contain glycerin. By means of the preparing method for the acellular corneal stroma, corneal cell components and soluble protein which are prone to causing the immune response can be effectively removed, and the complete acellular corneal stroma only containing the front elastic layer and the stroma layer can be obtained.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a decellularized corneal stroma and a preparation method thereof. Background technique [0002] Corneal disease is the second leading cause of blindness in the world, and the number of cases is increasing at a rate of 1.5 to 2 million per year. Corneal transplantation is currently the only effective way to treat corneal blindness, but the extreme scarcity of donor corneas restricts the development of corneal transplantation. Corneal stroma The corneal stroma of animals, such as animals, has similar tissue structure, biophysical properties and optical properties to human corneal stroma, but the strong rejection after xenotransplantation hinders the clinical application of xenogeneic corneas. Studies in recent years have found that the stromal cells in the corneal stroma are the main antigens that cause stromal rejection, and the collagen fibers that are the corneal stroma structure...

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 王维博张斌
Owner SHENZHEN AINEAR CORNEA ENG
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