Acellular corneal stroma and preparing method for acellular corneal stroma

Abstract

The invention discloses an acellular corneal stroma and a preparing method for the acellular corneal stroma. The preparing method for the acellular corneal stroma includes the following steps: (1) placing a picked animal cornea into water, and carrying out vibration processing to enable the epithelial layer to fall off; (2) replacing the water with acellular reagents, and carrying out vibration processing, wherein during the period, the acellular reagents and the water are alternately used to enable stroma cells and endothelial cells to fall off; (3) adding dehydrating agents, and enabling the mixture to stand or carrying out vibration processing to obtain the acellular cornea; (4) cutting the acellular cornea to obtain the acellular corneal stroma, wherein the acellular corneal stroma only comprises a front elastic layer and a stroma layer. The acellular reagents contain NaCl and EDTA, and the dehydrating agents contain glycerin. By means of the preparing method for the acellular corneal stroma, corneal cell components and soluble protein which are prone to causing the immune response can be effectively removed, and the complete acellular corneal stroma only containing the front elastic layer and the stroma layer can be obtained.

Description

Technical field [0001] The invention relates to the field of medical devices, in particular to an acellular corneal matrix and a preparation method thereof. Background technique [0002] Corneal disease is the second cause of blindness worldwide, and it is increasing at a rate of 1.5 to 2 million cases per year. Corneal transplantation is currently the only effective method to treat corneal blindness, but the extreme lack of donor corneal sources restricts the development of corneal transplantation. The corneal stroma, such as the corneal stroma of animals, has similar tissue structure, biophysical and optical properties to human corneal stroma, but the strong rejection after xenotransplantation hinders the clinical application of xenogeneic cornea. Recent studies have found that the stromal cells in the corneal stroma are the main antigens that cause stromal rejection. The collagen fibers, which are the framework of the corneal stroma, are highly conserved between germ lines an...

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Problems solved by technology

[0003] However, in the decellularization process of the acellular corneal stroma produced by the prior art, solutions such as trypsin, EDTA (ethylenediaminetetraacetic acid) and Triton-X100 (polyethylene glycol octylphenyl ether), deoxycholorin Sodium dodecyl sulfate, SDS (sodium dodecylsulfonate) and other surfactants, although these methods can remove cell components, but the reaction is severe, the corneal stroma is severely damaged, and good biocompatibility cannot be achieved

In addition, the acellular corneal stroma produced by the existing technology is a full-thickness cornea, which needs to be treated according to the size and depth of the wound before clinical use. During the operation, the probability of material contamination increases, and the stroma layer is easily damaged. The surface of the treated corneal stroma is uneven, which is not conducive to wound healing

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