Pig antimicrobial peptide NK-lysin fusion protein and encoding gene and application thereof

A technology of fusion protein and coding gene, which is applied in porcine antibacterial peptide NK-lysin fusion protein and its coding gene and application field, can solve problems such as multi-drug resistance and side effects of chemotherapy drugs, and achieve reduced production costs, high economic value, huge The effect of social benefits

Inactive Publication Date: 2014-08-27
SHANXI AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the problem of multi-drug resistance caused by the widespread use of traditional chemothe

Method used

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  • Pig antimicrobial peptide NK-lysin fusion protein and encoding gene and application thereof
  • Pig antimicrobial peptide NK-lysin fusion protein and encoding gene and application thereof
  • Pig antimicrobial peptide NK-lysin fusion protein and encoding gene and application thereof

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[0017] 1. Reagents used and their preparation

[0018] (1) LB (Luria-Bertani) liquid medium: Take 10g of peptone, 5g of yeast powder, 5g of NaCl, and dissolve in 800mL ddH 2 In O, adjust pH to 7.0 with 10M NaOH, dilute to 1000mL, autoclave (121℃, 1.034×10 5 Pa) Store at 4℃ for 20min.

[0019] (2) LB (Luria-Bertani) solid medium: per 1000mL ddH 2 Add 15g agar powder to O, add agar powder to LB liquid medium, autoclave and store at 4°C.

[0020] (3) Stock liquid:

[0021] 10×YNB (13.4%, containing ammonium sulfate but no amino acid): dissolve 34g YNB and 100g ammonium sulfate in 1000mL ddH 2 In O, filter sterilize and store at 4°C.

[0022] 500×Biotin (0.02%): 20mg biotin dissolved in 100mL ddH 2 In O, filter sterilize and store at 4°C.

[0023] 10×glucose (20%): 200g glucose dissolved in 1000mL ddH 2 In O, autoclave and store at 4°C.

[0024] 10 × methanol (5% methanol): 5mL methanol and 95mL ddH 2 O mix well, filter and sterilize, and store at 4°C.

[0025] 10×glycerol (10% glycerol): 100m...

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Abstract

The invention belongs to the field of gene engineering in the biotechnology pharmaceuticals industry, and particularly relates to pig antimicrobial peptide NK-lysin fusion protein and an encoding gene and application thereof. The amino acid sequence of the pig antimicrobial peptide NK-lysin fusion protein is shown in SEQIDNO: 2 in the specification. The advantages of antimicrobial peptide and the advantages of Pichiapastoris are utilized in the pig antimicrobial peptide NK-lysin fusion protein very well, the encoding gene of the pig antimicrobial peptide NK-lysin fusion protein can be efficiently shown in the Pichiapastoris, and the production cost of the antimicrobial peptide is reduced. The pig antimicrobial peptide NK-lysin fusion protein is applied to preparing medicines for inhabiting tumor cells, is a superior anti-cancer drug and has very high economic value and huge social benefits.

Description

technical field [0001] The invention belongs to the field of genetic engineering in the biotechnology pharmaceutical industry, in particular to a porcine antimicrobial peptide NK-lysin fusion protein and its coding gene and application. Background technique [0002] So far, the incidence and mortality of cancer are rising sharply. Although cancer research has paid a huge price, it has brought great difficulties to cancer treatment due to many reasons. Among them, the problem of multi-drug resistance caused by the widespread use of traditional chemotherapy drugs is one of the main reasons. In addition, chemotherapy drugs also have serious side effects. In recent years, studies have found that some antimicrobial peptides not only have antimicrobial activity, but also have a killing effect on tumor cells. Antimicrobial peptides are a class of biologically active small molecule polypeptides produced in organisms. They exist in a variety of organisms and are an important par...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/17A61P35/00
Inventor 姜俊兵武晓英王志瑞范阔海杜文娟郭夏斐
Owner SHANXI AGRI UNIV
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