Method for inducing nematode-trapping fungi to produce capturing devices through amino acid

A technology of preying on nematode fungi and preying organs, applied in the field of applied microbiology, can solve the problems of undiscovered public reports, etc., and achieve strong controllability and good repeatability

Inactive Publication Date: 2014-08-27
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through document search, do not find the public report

Method used

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  • Method for inducing nematode-trapping fungi to produce capturing devices through amino acid
  • Method for inducing nematode-trapping fungi to produce capturing devices through amino acid
  • Method for inducing nematode-trapping fungi to produce capturing devices through amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Valine (Val) induces the predatory nematode fungus Arthrobush oligosporum to produce predatory organs

[0047] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum oligospora blocks of the same size on the above CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.

[0048] According to the spore collection method described in the summary of the invention, collect the spores of Arthropodium oligospora, adjust the concentration of the spore suspension to about...

Embodiment 2

[0049] Example 2: Valine (Val) induces the nematode-predating fungus Arthrobus annuli to produce predatory organs

[0050] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum spp. blocks of the same size on the above-mentioned CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.

[0051] According to the method for collecting spores described in the summary of the invention, collect the spores of Arthrophyllum spp., adjust the concentration of the spore suspension to about 1...

Embodiment 3

[0052] Example 3: Leucine (Leu) induces the nematode-predating fungus Arthrophyllum oligosporum to produce predatory organs

[0053] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum oligospora blocks of the same size on the above CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.

[0054] According to the spore collection method described in the summary of the invention part, collect the spores of Arthropodium oligospora, adjust the concentration of the spore suspension...

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Abstract

The invention relates to a method for inducing nematode-trapping fungi to produce capturing devices through amino acid, and belongs to the field of microbiology application. The method comprises the steps of preparing an amino acid solution, cultivating the nematode-trapping fungi, collecting spores, soaking the spores in the amino acid solution and inducing the capturing devices. The method includes the specific steps that a, the amino acid used in amino acid solution preparation is valine or leucine or glutamic acid; b, conidia of the nematode-trapping fungi are adopted in the inducing step of the capturing devices, and the capturing devices are induced in a soaking mode through the amino acid solution. The method for inducing the nematode-trapping fungi to produce the capturing devices through the amino acid has the advantages that the spores of the nematode-trapping fungi are cultivated and collected, the valine or the leucine or the glutamic acid is used for soaking the spores, and the nematode-trapping fungi can be induced to produce a large number of capturing devices. The method is easy to operate and good in repeatability, a large number of capturing devices can be obtained, and a good experimental material is provided for further studying molecular mechanisms formed by the capturing devices of the nematode-trapping fungi.

Description

Technical field: [0001] The invention relates to a method for using amino acids to induce nematode-predating fungi to produce predatory organs, and belongs to the field of applied microbiology. Background technique: [0002] Plant parasitic nematodes are one of the important diseases of plants, and the annual economic loss of crops caused by plant parasitic nematodes is as high as 157 billion US dollars (Abad et al., 2008). In my country, nematodes harm almost all economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, wheat, rice, forest trees and Chinese medicinal materials, and become one of the important limiting factors in agricultural production. At present, the control of plant nematode diseases is still dominated by chemical control, but due to the impact of chemical pesticides on the ecological environment and the increase in the resistance of parasitic nematodes, its application is gradually limited, so the use of natural enemies of nematode...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01P5/00C12R1/645
Inventor 杨金奎苏浩赵勇张克勤
Owner YUNNAN UNIV
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