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Method of improving litter size of animals

A technology for litter size and animals, which is applied in the field of increasing the litter size of animals, and can solve the problem that the litter size of FSHR gene-transformed female mice has not been reported.

Inactive Publication Date: 2014-08-27
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, litter size was not reported for FSHR-transgenic dams compared to wild-type mice

Method used

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  • Method of improving litter size of animals
  • Method of improving litter size of animals
  • Method of improving litter size of animals

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, construction and identification of FSHR gene transgenic mice

[0052] 1. Construction of recombinant expression vector

[0053] synthesis figure 1 The pGH-A1366Gn plasmid is shown, and the sequence of the plasmid is shown in SEQ ID No.1.

[0054] The 690th to 695th from the 5' end of SEQ ID No.1 is the EcoRI restriction recognition site, and the 696th to 4213rd is the promoter sequence that can be specifically expressed on ovarian granulosa cells. Anti-Muller The promoter sequence (AMH) of vasculin hormone, No. 4214 to No. 6302 is the CDS sequence (pFSHR) of porcine FSHR gene, No. 6303 to No. 6527 is BGHpA (this sequence is a termination sequence), No. 6528 The 6533th position is the recognition site of Xho Ⅰ enzyme digestion.

[0055] figure 1 Among them, the target sequence composed of EcoRI recognition site, AMH, porcine FSHR gene CDS sequence (pFSHR), BGHpA and XhoI restriction site is as follows: figure 2 shown.

[0056] 2. Digest the pGH-A1366...

Embodiment 2

[0168] Embodiment 2, trans FSHR gene mouse and negative control mouse litter size statistics

[0169] 1. According to Table 2, the F0 generation mice were mated.

[0170] Table 2 F0 generation mouse mating situation

[0171]

[0172] The results in Table 2 show that the litter size of FSHR transgenic mice No. 16 and No. 33 is higher than that of negative control mice.

[0173] Two, carry out the mating of F1 generation mouse according to table 3

[0174] Table 3 F1 generation mouse mating situation

[0175]

[0176]

[0177] In Table 3, the F1 generation positive mice are the positive FSHR gene transgenic mice produced by mating the F0 generation positive FSHR gene mice in Table 2 with Kunming white mouse breed male mice, and the F1 generation negative mice are the F0 generation mice in Table 2 The non-transgenic FSHR gene mice produced by mating the positive transgenic FSHR gene mice with the Kunming white mouse breed, the identification process of the transgenic ...

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Abstract

The invention discloses a method of improving the litter size of animals. The invention verifies that the litter size of trans-FSHR (Follicle Stimulating Hormone Receptor)-genic mice is higher than that of non-transgenic mice. The method comprises the following steps: inserting the FSHR gene into an animal chromosome; and verifying to obtain the trans-FSHR-genic animals. Compared with the non-transgenic animals, the litter size of the trans-FSHR-genic animals is improved. The FSHR gene can promote improvement of the litter size of the mice.

Description

technical field [0001] The invention relates to a method for increasing the litter size of an animal litter, and belongs to the field of biotechnology. Background technique [0002] The general function of Follicle Stimulating Hormone (FSH) is to promote the development of female follicles and produce estrogen; or to regulate male Sertoli cells and spermatogenesis. Since FSH is a biological macromolecule and cannot penetrate the cell membrane, it must rely on the specific receptor on the cell membrane—follicle stimulating hormone receptor (FSHR) to function through the cAMP pathway. After the FSH signal acts on the ovarian tissue through FSHR, a series of physiological reactions such as hyperplasia of granulosa cells, differentiation of endometrial cells, formation of follicular fluid, and expansion of follicular cavity can occur, and play its biological function of promoting follicle growth. Superovulation is achieved under the action of high concentration of FSH and the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85A01K67/027C12N15/12
Inventor 储明星狄冉刘秋月王耀武杨志敏金慧慧
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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