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Method for implementing hypothermal anesthesia on fruit flies

A low-temperature anesthesia and fruit fly technology, applied in the fields of genetics and developmental biology, can solve the problems of no fruit flies, too fast awakening, unfavorable research and teaching activities of fruit flies, etc., to achieve rapid recovery, avoid safety hazards, and reduce difficulty Effect

Active Publication Date: 2014-09-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, when carrying out low-temperature anesthesia to fruit flies, it is necessary to first place the fruit fly anesthesia bottle in a low-temperature environment for a long time. After the fruit flies are anesthetized, they will wake up rapidly at room temperature (time is less than 120 seconds), and the fruit flies remain in the anesthetized state for too short time. Waking up too quickly is not conducive to scientific research and teaching activities with fruit flies as the object, so so far no low-temperature anesthesia technology and equipment for fruit flies have been put into scientific research and teaching

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] According to Table 1 and the specific operation time requirements after anesthesia of Drosophila, the selection of refrigerant and the calculation of refrigerant content are carried out according to the requirements of the ambient temperature of Drosophila hypothermia anesthesia. In this embodiment, ethanol-water mixture is selected as the refrigerant, and different minimum mass transfer temperatures of the refrigerant can be realized by adding different amounts of ethanol. The calculation formula is ΔT=-1.86×bB, wherein bB is the number of moles of ethanol added in one kilogram of water, ΔT is the theoretical minimum mass transfer temperature of the refrigerant, and the set low temperature anesthesia temperature (that is, the refrigerant provided by the fruit fly anesthesia bottle) Ambient temperature) is 2 to 3 degrees Celsius higher than the theoretical minimum mass transfer temperature of the refrigerant.

[0025] If 10.16 moles of ethanol are added to 1 kilogram of...

Embodiment 2

[0028] Table 2 shows the wake-up requirements of fruit flies that are immediately transferred to room temperature after being anesthetized at 0°C, -5°C, -10°C and -15°C for 600 seconds (10 minutes) and 1200 seconds (20 minutes). Time and 24-hour survival of Drosophila. As shown in Table 2, except for 0 degrees Celsius, although the awakening time of fruit flies at room temperature is prolonged after the storage temperature is lowered, the fruit flies also die with the reduction of the storage temperature, and the death of fruit flies is not conducive to fruit flies. for follow-up research on experimental subjects.

Embodiment 3

[0030] As shown in Table 1 and Table 2, no matter whether the fruit flies after anesthesia are transferred to room temperature immediately, or the fruit flies are anesthetized and kept for a certain period of time, neither can satisfy the follow-up research work with fruit flies as the experimental object (one One is the short wake-up time, one is the low survival rate of fruit flies).

[0031]Therefore, we have developed the new Drosophila anesthesia technique described in the present invention, that is, after the Drosophila is anesthetized at low temperature, it is placed in a low temperature environment of 0-5 degrees Celsius for a period of time (as shown in Table 3), and after subsequent observation and operation , and then placed in a room temperature environment to realize the awakening of fruit flies. Operators can choose the anesthesia temperature of fruit flies (-5 degrees Celsius, -10 degrees Celsius and -15 degrees Celsius), the low-temperature insulation temperatu...

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Abstract

The invention discloses a method for implementing hypothermal anesthesia on fruit flies, and belongs to the technical field of genetics and development biology. Through the method, high-biosecurity and environment-friendly anesthesia on the fruit flies which are import model organisms in the genetics can be achieved, anesthesia is simple in operation, anesthesia time of the fruit flies can be controlled, and the life span and fertility of the anesthetized fruit flies are not affected. The fruit flies are anesthetized at the environment temperature ranging from -15 DEG C to -5 DEG C for 25 seconds to 35 seconds, then the anesthetized fruit flies are transferred to the environment with the temperature ranging from 0 DEG C to 5 DEG C, and follow-up observation and operation of the fruit flies are finished within 20 minutes; eventually, the fruit flies are transferred to the indoor temperature environment, the fruit flies wake, and then follow-up culture and observation of the fruit flies are achieved. The environment temperature is controlled in a programming mode, and when the fruit flies are in the anesthetized state, it can be avoided that ice crystals are formed in the fruit flies due to the physical health due to the fact that the fruit flies are in the low-temperature environment for too long time, and then the life span and the fertility of the fruit flies are damaged.

Description

technical field [0001] The invention belongs to the technical field of genetics and developmental biology, and in particular relates to a method for implementing low-temperature anesthesia on fruit flies, through which the anesthesia of fruit flies, an important model organism in genetics, with high biological safety and environmental friendliness can be realized , the anesthesia operation is simple, the anesthesia time of the fruit fly is controllable, and the life span and reproductive ability of the fruit fly after anesthesia are not affected. Background technique [0002] Drosophila melanogaster, class Insecta, order Diptera, family Drosophila. It is one of the most thorough organisms currently studied by humans. It has the characteristics of fast reproduction, short generation time, and many offspring. The gene sequencing has been completed. It is an important model organism for genetics research. It is used in genetics experimental teaching and scientific research. ha...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 周杰张雪兰刘艳吕明孟令军权宇彤王亚文孟庆繁滕利荣
Owner JILIN UNIV
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