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Ractopamine molecular-imprinting piezoelectric sensor and preparation method and application thereof

A technology of ractopamine and piezoelectric sensors, applied in the direction of material electrochemical variables, etc., can solve the problem of limited number of effective imprinting sites, and achieve the effects of easy control of reaction conditions, good reproducibility, and good recognition selectivity

Inactive Publication Date: 2014-09-03
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polymer films synthesized in this way are usually dense, resulting in a limited number of effective imprinting sites formed during polymerization

Method used

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  • Ractopamine molecular-imprinting piezoelectric sensor and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Pretreatment of quartz crystal gold electrodes

[0026] Quartz crystal gold electrodes were first cleaned with ethanol and then immersed in "piranha" solution (30% H 2 o 2 :98%H 2 SO 4 =1:3, v / v) for 5 minutes, take it out and wash it with plenty of water and ethanol.

[0027] 2. Preparation of ractopamine molecularly imprinted piezoelectric sensor

[0028] (1) Add 50 μL of 0.5% ethanol solution of 1,6-hexanedithiol dropwise to the surface of the pretreated crystal gold electrode, self-assemble at room temperature for 2 hours, wash with ethanol, and then drop on the surface Add 50 μL prepared colloidal gold solution with a particle size of 17 nm, and self-assemble in the dark for 6 h. Then rinse carefully with water and dry for later use.

[0029] (2) Immerse the gold electrode self-assembled with gold nanoparticles on the surface in a concentration of 10mmol L -1 o-aminothiophenol ethanol solution overnight. The obtained electrode was first prepared with 10m...

Embodiment 2

[0032] 1. Pretreatment of quartz crystal gold electrodes

[0033] Quartz crystal gold electrodes were first cleaned with ethanol and then immersed in "piranha" solution (30% H 2 o 2 :98%H 2 SO 4 =1:3, v / v) for 5 minutes, take it out and wash it with plenty of water and ethanol.

[0034] 2. Preparation of ractopamine molecularly imprinted piezoelectric sensor

[0035] (1) Add 50 μL of 0.5% ethanol solution of 1,6-hexanedithiol dropwise to the surface of the pretreated crystal gold electrode, self-assemble at room temperature for 2 hours, wash with ethanol, and then drop on the surface Add 50 μL of prepared colloidal gold solution with a particle size of 17 nm, and self-assemble in the dark for 12 h. Then rinse carefully with water and dry for later use.

[0036] (2) Immerse the gold electrode self-assembled with gold nanoparticles on the surface in a concentration of 10mmol L -1 o-aminothiophenol ethanol solution overnight. The obtained electrode was first prepared with...

Embodiment 3

[0039] 1. Pretreatment of quartz crystal gold electrodes

[0040] The quartz crystal gold electrode was first cleaned with ethanol, then immersed in "piranha" solution (30%H2O2:98%H2SO4=1:3, v / v) for 5min, and rinsed with a large amount of water and ethanol after taking it out.

[0041] 2. Preparation of ractopamine molecularly imprinted piezoelectric sensor

[0042] (1) Add 50 μL of 0.5% ethanol solution of 1,6-hexanedithiol dropwise to the surface of the pretreated crystal gold electrode, self-assemble at room temperature for 2 hours, wash with ethanol, and then drop on the surface Add 50 μL prepared colloidal gold solution with a particle size of 20 nm, and self-assemble in the dark for 6 h. Then rinse carefully with water and dry for later use.

[0043] (2) The gold electrode self-assembled with gold nanoparticles on the surface was immersed in an ethanol solution of o-aminothiophenol at a concentration of 10 mmol L-1 overnight. The obtained electrode was first prepared i...

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Abstract

The invention relates to a ractopamine molecular-imprinting piezoelectric sensor and a preparation method thereof. The technical scheme is mainly technically characterized in that ractopamine is adopted as a template molecule, o-amino phenylthioalcohol is adopted as a functional monomer, an electropolymerization technology is adopted for synthesizing a poly-o-amino phenylthioalcohol film with high recognition selectivity to ractopamine on the surface of a quartz-crystal gold electrode self-assembled with gold nano particles, so that ractopamine can be quantitatively detected. The ractopamine molecular-imprinting piezoelectric sensor and the preparation method thereof have the beneficial effects that the characteristics such as high specific surface area of the gold nano particles, high selectivity of the molecular-imprinting polymer and high sensitivity of a quartz-crystal microbalance are effectively combined, the cost is low, the synthesis process is simple and the reaction conditions are easily controlled; and the prepared piezoelectric sensor has the advantages of high sensitivity, good reproducibility and long service life and the like, and can be applicable to determination of the content of the ractopamine in a feed sample.

Description

technical field [0001] The invention relates to a piezoelectric sensor based on molecular imprinting technology, in particular to a molecular imprinting piezoelectric sensor of ractopamine and its preparation method and application. Background technique [0002] Ractopamine is a typical β2 stimulant that can bind to β2 receptors on the cell membrane and is an artificial derivative of natural catechol. Because of its nutrient redistribution function, it can be used as a new type of growth-promoting additive in the production of livestock products. However, after hormone feed additives are used in animals, there is a problem of hormone residues. When ingested, it will cause poisoning, manifested as increased skeletal muscle contraction, destroying the fusion between fast-twitch muscle fibers and slow-twitch muscle fibers, causing muscle tremors, other symptoms of poisoning include tachycardia, arrhythmia, muscle pain, abdominal pain, dizziness and nausea, etc. . So far, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 王硕孔令杰王俊平方国臻
Owner TIANJIN UNIV OF SCI & TECH
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