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Non-woven material reinforced tissue engineering composite three-dimensional scaffold and preparation method thereof

A non-woven material and tissue engineering technology, applied in medical science, absorbent pads, prostheses, etc., can solve the problems of weak mechanical properties and easy damage of scaffolds, and achieve the effects of convenient processing, promotion of regeneration, and excellent biocompatibility

Inactive Publication Date: 2014-09-10
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, freeze-dried scaffolds have relatively weak mechanical properties and are easily damaged during use.

Method used

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  • Non-woven material reinforced tissue engineering composite three-dimensional scaffold and preparation method thereof
  • Non-woven material reinforced tissue engineering composite three-dimensional scaffold and preparation method thereof
  • Non-woven material reinforced tissue engineering composite three-dimensional scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The regenerated collagen short fibers were carded, laid, and reinforced by acupuncture to prepare a nonwoven material with an area density of 500 g / m2 and a thickness of 5 mm. Dissolve type A fish skin gelatin in deionized water to prepare gelatin solutions with concentrations of 2% and 5%. The regenerated collagen non-woven material is cut into a specified size and put into a plastic mold, and the gelatin solution is injected into the mold to completely immerse the non-woven material. The mold was placed in a -20°C refrigerator for 12 hours, and then freeze-dried using a freeze dryer. Utilize the alcohol solution of EDC / NHS to cross-link the gelatin, the cross-linking time is 12 hours, then wash with deionized water to remove the EDC / NHS, freeze-dry again, and obtain the gelatin three-dimensional scaffold material ( figure 2 ).

Embodiment 2

[0039] The regenerated collagen short fibers were carded, laid, and reinforced by acupuncture to prepare a nonwoven material with an area density of 500 g / m2 and a thickness of 5 mm. Dissolve 0.2 gram of A-type pigskin gelatin and 0.2 gram of silkworm regenerated silk fibroin in 10 ml of deionized water, heat and stir at 60 degrees for 2 hours to completely dissolve the gelatin and silk fibroin, and obtain a concentration of 4%, gelatin / silk fibroin mass ratio It is a 1:1 gelatin / silk fibroin solution. Measure 5 milliliters of gelatin and silk fibroin solution, add 0.05 gram of nano-hydroxyapatite particles therein, with a diameter of 80-120 nanometers, and disperse them evenly by ultrasonic vibration to obtain a nano-hydroxyapatite / gelatin / silk fibroin solution, wherein The mass ratio of each component is 1 / 2 / 2. The regenerated collagen non-woven material is cut into a specified size and put into a plastic mold, and the above two solutions are injected into the mold to compl...

Embodiment 3

[0041]The degummed silk was cut to obtain silk staple fibers, which were carded, laid, and spunlace reinforced to prepare a silk nonwoven material with an area density of 50 g / m2 and a thickness of 2 mm. Dissolve 0.5 g of Bombyx mori regenerated silk fibroin solution in 10 ml of deionized water, and stir to completely dissolve it to obtain a silk fibroin solution with a concentration of 5%. The silk non-woven material is cut into a specified size and put into a plastic mold, and the silk fibroin solution is injected into the mold to completely immerse the non-woven material. Place the mold in a -80 degree refrigerator for 12 hours, and then use a freeze dryer to dry for 48 hours. Soak the cross-linked silk fibroin in 95% alcohol solution for 2 hours, freeze-dry again to remove the alcohol and water, and obtain the silk fibroin three-dimensional scaffold material composited with the silk short fiber non-woven material.

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Abstract

The invention relates to a non-woven material reinforced tissue engineering composite three-dimensional scaffold and a preparation method thereof. The non-woven material reinforced tissue engineering composite three-dimensional scaffold is composed of a non-woven material reinforcer and a natural biomaterial free-dried scaffold. The preparation method comprises the following steps of (1) preparing fibers into the non-woven material by use of the non-weaving technology; (2) immersing the non-woven material in the prepared aqueous solution of the natural biomaterial; (3) removing moisture by use of the free-drying technology; (4) immersing the natural biomaterial in a crosslinking agent, thereby obtaining the non-woven material reinforced tissue engineering composite three-dimensional scaffold. The preparation method of the non-woven material reinforced tissue engineering composite three-dimensional scaffold is simple to operate, high in stability, free from use of an organic solvent in the whole process, and environmental friendly and safe; the prepared three-dimensional tissue engineering composite scaffold is excellent in mechanical properties; the fibers in the non-woven material and the natural biomaterial part in the scaffold are closely bonded together, so that the structural stability of the scaffold is effectively improved and the mechanical properties of the scaffold are enhanced.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and biomanufacturing, and in particular relates to a non-woven material reinforced tissue engineering composite three-dimensional support and a preparation method thereof. Background technique [0002] One of the three elements of tissue engineering is the preparation of three-dimensional scaffold materials. The introduction of suitable scaffold materials and three-dimensional structure is an important factor to promote the differentiation of cells in the desired direction to form tissues to repair wounds. The three-dimensional porous cell scaffold not only provides support for cell adhesion, but also creates necessary conditions for the delivery of nutrients and the discharge of metabolic waste. Its shape and structure directly affect the shape and function of the formed tissue. [0003] Natural biomaterials, such as gelatin, collagen, silk fibroin, polysaccharides, etc., are natural ...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/54A61L27/56A61L27/58A61L15/32A61L15/28A61L15/26A61L15/44
Inventor 柯勤飞李大伟莫秀梅黃晨
Owner DONGHUA UNIV
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