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Method for quickly measuring content of multiple index components in radix ophiopogonis through near infrared spectroscopy

A near-infrared spectroscopy and near-infrared spectrometer technology is applied in the field of quality inspection of Ophiopogon japonicus, and can solve the problems of high production cost, waste of raw materials, chemical pollution and the like

Active Publication Date: 2014-09-17
常熟雷允上制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Purpose of the invention: the purpose of the present invention is to overcome the deficiencies in the detection of Ophiopogon japonicus active ingredients in the prior art, and provide a method for quickly and simultaneously measuring the contents of total saponins, total flavonoids, and total polysaccharides in Ophiopogon japonicus using near-infrared spectroscopy. The present invention provides The method can effectively solve the problems of cumbersome traditional Chinese medicine quantitative analysis methods, high production costs, low efficiency, excessive waste of raw materials, and serious chemical pollution.

Method used

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  • Method for quickly measuring content of multiple index components in radix ophiopogonis through near infrared spectroscopy
  • Method for quickly measuring content of multiple index components in radix ophiopogonis through near infrared spectroscopy
  • Method for quickly measuring content of multiple index components in radix ophiopogonis through near infrared spectroscopy

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Effect test

Embodiment 1

[0088] 1, a kind of method that adopts near-infrared spectrum to measure the multi-index component content in Ophiopogon japonicus quickly, it comprises the following steps:

[0089] (1) Collection and pretreatment of Ophiopogon japonicus samples: Collect 91 parts of Ophiopogon japonicus medicinal materials, store them in low-temperature freezer for 48-72 hours, take out and pulverize the coarse powder, dry at 60°C for 24 hours, and then continue to pulverize into fine powder, pass through an 80-mesh medicine sieve, Placed in a desiccator as a sample of Ophiopogon japonicus;

[0090] (2) Collection of original spectra: 61 samples of Radix Ophiopogon japonicus were taken as the calibration sample set, and 30 samples were used as the verification sample set to ensure that the content distribution of each component of the calibration sample had a certain range, and an appropriate amount of fine powder of medicinal materials was put into the quartz sample In the cup, mix evenly, u...

Embodiment 2

[0113] The method for the rapid determination of total flavonoid content in Ophiopogon japonicus by near-infrared spectroscopy comprises the following steps:

[0114] (1) Collection and pretreatment of Ophiopogon japonicus samples: Collect 91 parts of Ophiopogon japonicus medicinal materials, store them in low-temperature freezer for 48-72 hours, take out and pulverize the coarse powder, dry at 60°C for 24 hours, and then continue to pulverize into fine powder, pass through an 80-mesh medicine sieve, Placed in a desiccator as a sample of Ophiopogon japonicus;

[0115] (2) Collection of original spectra: 61 samples of Radix Ophiopogon japonicus were taken as the calibration sample set, and 30 samples were used as the verification sample set to ensure that the content distribution of each component of the calibration sample had a certain range, and an appropriate amount of fine powder of medicinal materials was put into the quartz sample In the cup, mix evenly, use air as a refe...

Embodiment 3

[0141] The method for the rapid determination of total polysaccharide content in Radix Ophiopogon japonicus by near-infrared spectroscopy comprises the following steps:

[0142] (1) Collection and pretreatment of Ophiopogon japonicus samples: Collect 91 parts of Ophiopogon japonicus medicinal materials, store them in low-temperature freezer for 48-72 hours, take out and pulverize the coarse powder, dry at 60°C for 24 hours, and then continue to pulverize into fine powder, pass through an 80-mesh medicine sieve, Placed in a desiccator as a sample of Ophiopogon japonicus;

[0143] (2) Collection of original spectra: 61 samples of Radix Ophiopogon japonicus were taken as the calibration sample set, and 30 samples were used as the verification sample set to ensure that the content distribution of each component of the calibration sample had a certain range, and an appropriate amount of fine powder of medicinal materials was put into the quartz sample In the cup, mix evenly, use ai...

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Abstract

The invention discloses a method for measuring the content of multiple index components in radix ophiopogonis. The method comprises the steps of performing scanning through a near infrared spectrometer, acquiring original near infrared spectroscopy data, and preprocessing the original near infrared spectroscopy data through TQAnalyst spectroscopic analysis software so as to obtain near infrared spectroscopy data for the content of the index components in the radix ophiopogonis; measuring the content of the index components in the radix ophiopogonis in a correction set through a spectrophotometric method, combining the content with feature spectroscopic information for the content measurement of the index components in the radix ophiopogonis in a correction sample set, respectively constructing correction models for the content measurement of the index components in the radix ophiopogonis by a partial least square method, substituting a checking sample set feature spectrum into the correction models to obtain an NIR (near infrared) forecast value, comparing the NIR forecast value with the content of the components in a checking sample set measured by the conventional method so as to check the correction models, and constructing a quantification model for the multiple index components in the radix ophiopogonis. The method disclosed by the invention has the advantages of short analysis time, high speed, accurate analysis result and the like, and has important significance.

Description

technical field [0001] The invention relates to a quality detection method of Ophiopogon japonicus, in particular to a method for rapidly measuring the content of multiple index components in Ophiopogon japonicus by using near-infrared spectroscopy. Background technique [0002] Ophiopogon japonicus (Thunb.) Ker-Gawl is the dried tuber root of Liliaceae Ophiopogon japonicus (Thunb.) Ker-Gawl. It is a commonly used traditional Chinese medicine for nourishing yin. It was first recorded in "Shen Nong's Materia Medica". The efficacy of this medicine is mainly used to treat diseases such as dryness of the lungs, dry cough, and thirst for fluid wounds. Its main components are steroidal saponins, polysaccharides, high isoflavones, amino acids and lipids, etc. Among them, Ophiopogon japonicus saponins, flavonoids and polysaccharides are the main medicinal active ingredients of Ophiopogon japonicus, which have anti-myocardial ischemia, anti-hypoxia, immune activity, Pharmacological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563G01N1/28
Inventor 王欢顾治平王恒斌陈力建
Owner 常熟雷允上制药有限公司
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