A nano-silver colorimetric method for detecting copper ions
A technology of nano silver and copper ions is applied in the field of chemical analysis and detection, which can solve the problems of high cost and long detection time, and achieve the effects of low cost, convenient detection method and good selectivity.
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Embodiment 1
[0028] A nano-silver colorimetric method for detecting copper ions specifically comprises the following steps:
[0029] (1) A bright yellow nano-silver solution is prepared by reducing silver nitrate with sodium citrate;
[0030] (2) Add the solution to be tested containing copper ions to the aqueous solution containing 1,4-dithiothreitol (DTT) and 2,6-pyridinedicarboxylic acid (PDCA), and mix well to obtain solution A;
[0031] (3) Incubate solution A at a temperature of 10-60°C for 5-60 min to obtain solution B;
[0032] (4) Add the solution B obtained in step (3) to the nano-silver solution in step (1), mix well, and the nano-silver aggregates to different degrees. E 408 / E 650 As the ordinate, and the copper ion concentration as the abscissa, draw the standard curve of copper ions.
[0033] Step (2) The concentration of 1,4-dithiothreitol (DTT) in solution A is 0.5-10 mmol / L, and the concentration of 2,6-pyridinedicarboxylic acid (PDCA) in solution A is 0.5-5 mmol / L; ...
Embodiment 2
[0036] Preparation of silver nanoparticle solution: Dissolve 17 mg of silver nitrate in 100 ml of distilled water and transfer it to a 250 ml clean three-necked flask, heat it to boiling, add 2 ml of 2% sodium citrate solution, and continue heating under magnetic stirring Boiled for 30min, naturally cooled to room temperature to obtain silver nano-solution for later use. like figure 1 As shown, the diameter of the prepared silver nanoparticles is about 25 nm, the color of the solution is bright yellow, and the silver nanoparticles are in a monodispersed state.
[0037] Take 10 ml of the silver nano-solution prepared above, add 100 μL of DTT solution with a concentration of 1 mmol / L, and place it at room temperature 25 ° C for 10 min, such as figure 2 As shown, the solution turned to a dark green solution. The dark green solution is centrifuged at 8000-10000 r / min for 10-20min, the bottom of the centrifuge is added dropwise to the copper mesh, dried and tested to obtain a tr...
Embodiment 3
[0041] The copper ion test solution containing 1,4-dithiothreitol (DTT) at a concentration of 0.5 mmol / L and 2,6-pyridinedicarboxylic acid (PDCA) at a concentration of 0.5 mmol / L was placed at 10°C Incubate at high temperature for 5 minutes; then add the nano-silver solution to the incubated solution to be tested, the volume ratio of the solution to be tested and the nano-silver solution is 1:50, mix well, and detect the ultraviolet-visible absorption spectrum of the solution after 5 minutes to obtain Absorbance ratio E 408 / E 650 As the ordinate and the copper ion concentration as the abscissa, a standard curve of copper ions is drawn. Others are identical with embodiment 2.
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