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Transfer faction solution and preparation method thereof

A transfer factor and solution technology, which is applied in the field of transfer factor solution and its preparation, can solve the problems of low content, low yield of transfer factor solution, and high content of polymer substances, so as to improve product yield, increase stability and safety , the effect of increasing the content

Active Publication Date: 2014-10-15
卫材(辽宁)制药有限公司
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the content of polypeptides, free amino acids, and nucleotides in the transfer factor solution prepared by the prior art is relatively low, the content of high molecular substances is relatively high, and the yield of the transfer factor solution is low.

Method used

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  • Transfer faction solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of transfer factor solution (see figure 1 ):

[0022] Rinse the thawed pig spleen with purified water, remove the fat, cut off the connective tissue, put it into a meat grinder, and mince it (2-3mm) for later use.

[0023] The minced spleen is mixed with 0.9% NaCl (W / V) (W / V, mass volume ratio) solution at a weight ratio of 1:1.3, and then added to a colloid mill to grind for 1 to 2 minutes to obtain a uniform Slurry, wherein the gap of the colloid mill is 5-10um.

[0024] The homogenate was subjected to repeated freezing and thawing for 5 times. After freezing and thawing, use 2mol / L HCL solution to mix the homogeneous slurry to a pH value of 4.0, and then centrifuge at a speed of 2000 rpm for 30 minutes to collect the supernatant. L of NaOH solution to adjust the pH value to 7.0, adjust the pH value and then filter. After filtration, the filtrate is ultra-filtered through a hollow fiber column, and the filtrate is the transfer factor solution....

Embodiment 2

[0028] The preparation method of transfer factor solution (see figure 1 ):

[0029] Rinse the thawed pig spleen with purified water, remove the fat, cut off the connective tissue, put it in a meat grinder, and grind it for use.

[0030] After mincing, the spleen is mixed with 0.9% NaCl solution at a weight ratio of 1:1.4, and then added to a colloid mill to grind for 1 to 2 minutes to obtain a homogenate. Among them, the gap of the colloid mill is 5-10um.

[0031] The homogenate was subjected to repeated freezing and thawing for 5 times. After freezing and thawing, use 2mol / L HCL solution to mix the homogeneous slurry to a pH value of 4.0, then centrifuge at a speed of 2500 rpm for 20 minutes, collect the supernatant, and then pass the supernatant through 2mol / L The NaOH solution of 1 L adjusts the pH value to 7.0, and filters after adjusting the pH value. After filtration, the filtrate is ultra-filtered through a hollow fiber column (pressure is controlled at 0.1-0.2Mpa), a...

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Abstract

The invention belongs to the technical field of biological pharmaceutical manufacturing, and particularly relates to a transfer faction solution and a preparation method thereof. The preparation method comprises the following steps: mixing animal spleen and NaCl solution according to a weight ratio of 1:(1.3-1.4), performing repeated freeze-thaw, adjusting the pH value to 6.2-7.2, and filtering to obtain the transfer faction solution. By the adoption of the method provided by the invention, the content of effective components in the transfer faction solution can be increased advantageously, the product yield is improved, and the stability and the safety of a medicine are improved.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a transfer factor solution and a preparation method thereof. Background technique [0002] Transfer factor solution is a natural two-way immunomodulator, and it is an ideal drug for treating related diseases such as low immune function and deficiency. [0003] The transfer factor solution is a mixture of polypeptides, amino acids and polynucleotides with a molecular weight of less than 6000 Daltons, which is made from healthy pig or bovine spleen as raw material, fat-removing, cell crushing, dialysis or ultrafiltration. [0004] However, the content of polypeptides, free amino acids, and nucleotides in the transfer factor solution prepared in the prior art is relatively low, the content of macromolecular substances is relatively high, and the yield of the transfer factor solution is low. Contents of the invention [0005] The purpose of the present invention is to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P37/02
Inventor 贾天亮郑桂荣梁爱葵
Owner 卫材(辽宁)制药有限公司
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