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Crocodile lecithin preparation method

A technology of crocodile lecithin and crocodile, applied in the direction of edible phospholipid composition, food science, protein food ingredients, etc., can solve the problems of less lecithin extract, high cost of industrial application equipment, and can only be carried out in laboratories, etc., to achieve high efficiency effect

Inactive Publication Date: 2014-10-15
汪华东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the Chinese patent 201210384071.7 "A Method for Extracting Egg Yolk Lecithin", it passes egg yolk powder through supercritical CO 2 Egg oil is removed by fluid extraction to obtain deoiled egg yolk powder; secondly, the deoiled egg yolk powder is extracted with ethanol and filtered to obtain an ethanol extract; the acid value of the ethanol extract is measured, and aluminum oxide is added to the ethanol extract according to the acid value result. Adsorption treatment; finally, the ethanol adsorption solution is filtered and dried to obtain the finished product egg yolk lecithin, but when the method is applied to the lecithin extraction of crocodile eggs, it is found that the lecithin extract is less, and the supercritical CO 2 Fluid extraction can only be carried out in the laboratory, and the cost of industrial application equipment is high. Therefore, it is necessary to develop a preparation method for crocodile lecithin with high extraction efficiency on the basis of existing equipment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1: Take 100g of crocodile egg yolk from Tai crocodile, add it to 80g of water, add 0.6g of a mixture of pepsin and trypsin, stir for 4 hours, and control the temperature at 20±1°C;

[0023] Step 2: first rough extraction, add 250g of 96% ethanol and 120g of analytically pure dichloromethane to the mixture obtained in step 1, stir for 25 minutes, filter with suction, collect the filter residue and filtrate, and let the filtrate stand Separation of oil layer by layer;

[0024] Step 3: second rough extraction, add 250g of 97% ethanol and 90g of analytically pure dichloromethane to the filter residue obtained in step 2, stir for 15 minutes, suction filter, collect filter residue and filtrate, and let the filtrate stand Separation of oil layer by layer;

[0025] Step 4: For the third rough extraction, add 250g of 98% ethanol and 70g of dichloromethane to the filter residue obtained in step 3, stir for 10 minutes, filter with suction, collect the filter residue and filt...

Embodiment 2

[0029] The specific method is the same as in Example 1, except that the enzymolysis time is 7 hours and the weight of the enzyme is 0.2 g.

Embodiment 3

[0031] The specific method is the same as in Example 1, except that the weight of ethanol in steps 2, 3, and 4 is 200 g.

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Abstract

The invention discloses a crocodile lecithin preparation method which comprises the steps of enzymolysis, first coarse extraction, second coarse extraction, third coarse extraction, solvent removal in a pressure reduction manner, drying and compression. The invention aims to provide the crocodile lecithin preparation method with high efficiency.

Description

technical field [0001] The invention relates to the field of crocodile products, in particular to a preparation method of crocodile lecithin. Background technique [0002] Crocodile is a kind of ancient reptile, "Compendium of Materia Medica" and other ancient medical classics have records about the medicinal value of crocodile. As early as the Southern and Northern Dynasties, there is a record of the crocodile carapace in the "Compendium of Materia Medica": "It tastes pungent and slightly warm. It is mainly associated with abdominal pain, phlegm, accumulation, cold and heat." Internal symptoms". The forty-third volume of "Scales" in Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty recorded in detail: "The crocodile (that is, crocodile) armor is mainly used for the treatment of confidant's abdominal pain; Record dampness and evil, all kinds of internal symptoms in the vomitus, and malignant blisters. Fat: Indications for rubbing wind and malignant sores." In...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 汪华东
Owner 汪华东
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