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Application of KAP26.1 gene as exogenous gene introduced into cashmere goat cells and used for improving wool fineness

A KAP26.1, exogenous gene technology, applied in the direction of plant gene improvement, application, genetic engineering, etc.

Inactive Publication Date: 2014-10-15
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Those skilled in the art know that exogenous genes are DNA sequences introduced into target cells through genetic engineering techniques or virus infection, but so far, there is no information about using KAP26.1 gene as exogenous genes through genetic engineering techniques or Reports on the introduction of cashmere goat cells by virus infection and other ways to improve the fineness of cashmere

Method used

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  • Application of KAP26.1 gene as exogenous gene introduced into cashmere goat cells and used for improving wool fineness
  • Application of KAP26.1 gene as exogenous gene introduced into cashmere goat cells and used for improving wool fineness
  • Application of KAP26.1 gene as exogenous gene introduced into cashmere goat cells and used for improving wool fineness

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Experimental program
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Embodiment Construction

[0014] 1. Primary skin cell culture of Liaoning cashmere goat

[0015] (1) Select about 5cm from the buttocks of Liaoning cashmere goats 2 Use wool shears to cut off the long hair and velvet, disinfect with iodine, and then use 75% alcohol to remove the code, and cut about 0.5cm with a blade. 3 Immediately put tissue pieces of the same size into 75% alcohol, wash for 30s, rinse three times with saline containing 100U / ml double antibody, and finally put sheepskin tissue pieces into D-Hanks, seal with parafilm, and store at 4°C Save and bring back to the laboratory within 4 hours.

[0016] (2) Place the small piece of sheep skin in D-Hanks buffer, rinse it three times with D-Hanks solution, and then cut it into 1mm with ophthalmic scissors 3 Small tissue blocks left and right. Take small pieces of tissue and place them in the culture bottle and evenly distribute them at the bottom of the culture bottle. After the left and right sides are completed, the bottom of the bottle i...

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Abstract

The invention discloses an application of a KAP26.1 gene as an exogenous gene introduced into cashmere goat cells and used for improving wool fineness due to the fact that the KAP26.1 gene produces a regulating effect on Liaoning cashmere goat wool fineness, that is, the higher the expression quantity of the KAP26.1 gene is, the smaller the wool fineness is, meanwhile, the high expression of the KAP26.1 gene can prompt increase of expression quantity of a KAP7.1 gene, KAP11.1 gene and the like, the wool fineness can be reduced with the gene increase, and accordingly, the wool quality is improved. According to the application, KAP26.1 gene overexpression lentivirus is injected into the testis of a Liaoning cashmere goat, after the KAP26.1 gene is taken as the exogenous gene to be introduced into the Cashmere goat cells through virus infection, a Liaoning cashmere goat individual with high KAP26.1 gene expression quantity is selected and bred as a breeding goat, so that the cashmere goat wool fineness can be improved, and the wool quality is increased.

Description

technical field [0001] The invention relates to a new application of KAP26.1 gene, in particular to the application of KAP26.1 gene as an exogenous gene introduced into cashmere goat cells to improve the fineness of cashmere. Background technique [0002] Cashmere goat hair consists of coarse hairs produced by primary hair follicles and down hairs produced by secondary hair follicles. Velvet has slenderness, lightness, smoothness, warmth, soft luster, high elasticity, excellent textile performance and other characteristics incomparable to other fibers. It is the most precious natural fiber raw material produced by the world's textile industry and is known as "soft gold". Improving the quality of fluff and increasing the output of fluff are the two main indicators to improve the economic value of cashmere goats, and the fineness of fluff is the main index to measure the quality of fluff, that is, the finer the fluff, the better the quality. In 2011, Jinmei et al. found that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867A01K67/027
Inventor 金梅赵凤琴朴敬爱朴君李秀娜
Owner LIAONING NORMAL UNIVERSITY
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