The method for increasing the production of rhamnolipid and its specific Pseudomonas aeruginosa

A technology of Pseudomonas aeruginosa and rhamnolipid, which can be applied in the biological field and can solve problems such as unclear chemical structure
CN104099388BActive Publication Date: 2016-09-21INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2016-09-21

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Abstract

The invention discloses a method for preparing rhamnolipid and its special Pseudomonas aeruginosa. The method for preparing rhamnolipids includes 1) and 2): 1) constructing a recombinant Pseudomonas aeruginosa with reduced Psl polysaccharide synthesis ability and / or reduced Pel polysaccharide synthesis ability compared with Pseudomonas aeruginosa as the starting bacterium 2) fermenting the recombinant Pseudomonas aeruginosa to obtain rhamnolipids. The experiment of the present application proves that compared with Pseudomonas aeruginosa as the starting bacterium, the rhamnolipid synthesis ability of recombinant Pseudomonas aeruginosa with reduced Psl polysaccharide synthesis ability and / or reduced Pel polysaccharide synthesis ability is improved.
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Description

technical field

[0001] The invention relates to a method for increasing the yield of rhamnolipid in the field of biotechnology and the special Pseudomonas aeruginosa. Background technique

[0002] Rhamnolipid is a glycolipid anionic surfactant. It has good biocompatibility and efficient emulsification, solubilization and surface tension reduction capabilities, and has broad application prospects in petroleum exploration, biomedicine, environmental protection and food and other fields.

[0003] At present, the production strain of rhamnolipid is mainly Pseudomonas aeruginosa, and the improvement of its production performance is generally through various physical and chemical mutagenesis techniques to improve the production capacity of the strain rhamnolipid, and physical and chemical mutagenesis is very easy to reverse the mutation , resulting in unstable production performance of the strain, and a new method for constructing a rhamnolipid high-yielding strain is urgently ne...

Claims

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