Culture method of pasty photosynthetic bacteria
A technology of photosynthetic bacteria and culture methods, applied in the field of breeding, can solve problems such as difficult bottom sinking, low bacterial count, and difficult storage
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Embodiment 1
[0014] Take the Rhodopseudomonas palustris bacteria liquid, take 25% of the volume of the culture solution, inoculate it in the culture solution, cultivate it at 25°C, and cultivate it for 5 days under the light intensity of 3000LX; take 50% of the volume, and transfer it to a new In the culture medium, the culture temperature is 25°C, under the light intensity of 3000LX, after culturing for 5 days, take 50% of the volume, and transfer it to a new culture medium, at the culture temperature of 25°C, under the light intensity of 3000LX, after culturing for 5 days, use 10% acetic acid, adjust the pH to 2, continue to cultivate for 5 days, complete the cultivation process, and obtain the required paste-like photosynthetic bacteria.
[0015] Culture solution composition: yeast extract 0.2g, sodium acetate 3.0g, sodium bicarbonate 1.0g, ammonium chloride 1.0g, sodium chloride 5.0g, magnesium chloride 0.2g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.2g, Cal...
Embodiment 2
[0017] Take Rhodopseudomonas palustris bacteria liquid, take 30% of the volume of the culture solution, inoculate it in the culture solution, cultivate at a temperature of 30°C, and cultivate for 4 days under the light intensity of 2000LX; take 50% of the volume, and transfer it to a new In the culture medium, the culture temperature is 30°C, under the light intensity of 2000LX, after 4 days of culture, take 50% of the volume, and transfer it to a new culture medium, the culture temperature is 35°C, under the light intensity of 2000LX, after 4 days of culture, use 10% acetic acid, adjust the pH to 7.5, and continue to cultivate for 5 days to complete the cultivation process.
[0018] Culture solution composition: yeast extract 0.05g, sodium acetate 3.0g, sodium bicarbonate 1.0g, ammonium chloride 1.0g, sodium chloride 5.0g, magnesium chloride 0.2g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.2g, Calcium chloride 0.05g, ferrous chloride-EDTA 0.005g, vi...
Embodiment 3
[0020] Take the Rhodopseudomonas sphaeroides bacteria liquid, take 25% of the volume of the culture solution, inoculate it in the culture solution, cultivate it at a temperature of 30°C, and cultivate it for 5 days under the light intensity of 2000LX; take 50% of the volume, and transfer it to a new In the culture medium, the culture temperature is 30°C, under the light intensity of 2500LX, after 5 days of culture, take 50% of the volume, and transfer it to a new culture medium, the culture temperature is 30°C, under the light intensity of 2000LX, after 5 days of culture, use 15% acetic acid, adjust the pH to 7.3, continue to cultivate for 5 days, complete the cultivation process, and obtain the required paste-like photosynthetic bacteria.
[0021] Culture medium composition: yeast 15g, sodium acetate 3.2g, sodium bicarbonate 1.5g, ammonium chloride 1.0g, sodium chloride 5.0g, magnesium chloride 0.2g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.2g, chl...
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