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Genetic marker for schizophrenia as well as using method and application of genetic marker

A schizophrenia and genetic technology, applied in the medical field, can solve problems such as economic burden and long-term recurrent attacks

Active Publication Date: 2014-10-29
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While some patients recover with treatment, most suffer from recurrent episodes over a long period of time, making schizophrenia not only an individual disease but also a serious social problem with consequences for families, the health care system and society at large obvious financial burden

Method used

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  • Genetic marker for schizophrenia as well as using method and application of genetic marker
  • Genetic marker for schizophrenia as well as using method and application of genetic marker
  • Genetic marker for schizophrenia as well as using method and application of genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0149] The amplification curves of real-time quantitative PCR of genes ZNF184, ZSCAN12 and HIST1H4I and internal reference gene ACTB are as follows: image 3 Shown (shows an overlay of 20 amplification curves for each gene, including 10 samples). The shapes of the curves are relatively normal, and the threshold setting (green horizontal line) meets the basic requirements. The expression (Ct value) of the Beta-actin gene (ACTB) in different samples is basically stable, which is in line with its characteristics as an internal reference gene. The expression value of the ZNF184 gene in one of the normal samples did not meet the data screening requirements and was treated as a missing value. The expression of the three genes in the training set samples is shown in the box plot ( Figure 4 ), their expression levels were all reduced in patients.

[0150] Using the Student's t-test to analyze the differences of the three genes in the two groups, the results are shown in Table 2, an...

Embodiment 2

[0164] Example 1 was repeated, but using the genes ZNF184, ZSCAN12 and HIST1H4I each and combinations in between.

[0165] The linear equations used for diagnosis are constructed in the model training set using logistic regression, where "P" represents the probability of disease, 1 is diseased, 0 is not diseased; "c (gene)" means that the gene is in Relative expression levels measured in blood leukocytes.

[0166] The diagnostic specificity / sensitivity lookup tables for each diagnostic marker and their pairwise combinations were obtained based on all samples, and the specific lookup table is shown below.

[0167] Table 4. Diagnostic specificity / sensitivity query table for ZNF184 alone

[0168] Y=c(ZNF184)

[0169]

[0170]

[0171]

[0172] Table 5. Diagnostic specificity / sensitivity query table for ZSCAN12 alone

[0173] Y=c(ZSCAN12)

[0174]

[0175]

[0176]

[0177] Table 6. Diagnostic specificity / sensitivity query table for HIST1H4I alone

[0178] Y...

Embodiment 3

[0208] According to the criteria described in the "Materials and Methods" section, 30 Changsha patients with schizophrenia and 30 cases of control group were selected for the first episode or after long-term drug withdrawal; the above samples were subjected to double-blind detection to detect ZNF184 and / or ZSCAN12 and And / or the expression level of the HIST1H4I gene, and then use the linear equation and the threshold value provided in Examples 1 and 2 to diagnose whether the test subject suffers from schizophrenia.

[0209] result:

[0210] Using the expression value of ZNF184 gene alone, the detection accuracy is 78.5% (sensitivity is 76%, specificity is 81%, threshold value is 4.56*E-04); using ZSCAN12 alone, the detection accuracy is 83.4% (sensitivity is 79.3 %, the specificity was 87.5%, and the threshold was 3.95*E-04); with HIST1H4I alone, the detection accuracy was 80.8% (the sensitivity was 82.8%, the specificity was 78.8%, and the threshold was 5.57*E-04).

[0211] ...

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Abstract

The invention discloses a group of marker genes which can be used for diagnosing schizophrenia. The marker genes can be used for preparing a detection reagent or a detection kit for diagnosing schizophrenia. Based on the marker genes, the invention further provides the detection kit, the detection reagent and a gene chip or an array for diagnosing schizophrenia, as well as a method for diagnosing schizophrenia by utilizing the marker genes. The method disclosed by the invention is simple and easy to implement, and the diagnosed results are reliable, and meet needs of sensitivity and specificity.

Description

technical field [0001] The present invention relates to the field of medicine. In particular, the present invention relates to gene markers for schizophrenia, methods of use and applications thereof. Background technique [0002] Schizophrenia is a common mental illness with a prevalence of 0.3%-0.7% in the global population [1]. Schizophrenia usually occurs in young and middle-aged adults, and there are often obstacles in perception, thinking, emotion, and behavior, as well as incoordination of mental activities, which seriously affect the patient's physical function and quality of life. While some patients recover with treatment, most suffer from recurrent episodes over a long period of time, making schizophrenia not only an individual disease but also a serious social problem with consequences for families, the health care system and society at large obvious financial burden. [0003] At present, the clinical diagnosis of schizophrenia is mainly based on the patient's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B40/06
CPCC12Q1/6883C12Q2600/158
Inventor 万春玲孙丽雅青颖杨仑
Owner SHANGHAI JIAO TONG UNIV
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