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Method for distinguishing wild radix astragali from cultured radix astragali

A technology for cultivating Astragalus membranaceus and wild Astragalus membranaceus, which is applied in the field of identification of wild Astragalus membranaceus and cultivated Astragalus membranaceus, and can solve the problems of difficult identification of Astragalus membranaceus medicinal materials and inability to accurately distinguish wild and cultivated Astragalus membranaceus

Active Publication Date: 2014-10-29
SHANXI UNIV
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wild Astragalus membranaceus is different from cultivated Astragalus membranaceus in appearance. Experienced people can judge the growth mode of Astragalus membranaceus through the appearance and shape of Astragalus membranaceus, such as length and diameter, but it is difficult to judge the astragalus membranaceus after being crushed.
In the Chinese Pharmacopoeia, the content of astragaloside IV and calycosin glucoside is used for the quality control of Astragalus membranaceus, but the existing quality control indicators of flavonoids and saponins cannot accurately distinguish wild and cultivated Astragalus membranaceus

Method used

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  • Method for distinguishing wild radix astragali from cultured radix astragali
  • Method for distinguishing wild radix astragali from cultured radix astragali
  • Method for distinguishing wild radix astragali from cultured radix astragali

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Experimental program
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Embodiment 1

[0016] Embodiment 1, determination of wild and cultivated Radix Astragali SI value

[0017] Astragalus samples from different sources were collected, including 66 batches of wild Astragalus and 45 batches of cultivated Astragalus, see Table 1 for details.

[0018] Table 1 Information of Astragalus samples from different sources

[0019]

[0020] 1) Sample preparation: Weigh 200 mg of sample powder and place it in a 10 mL glass centrifuge tube, add 6 mL of 50% methanol for ultrasonic extraction, extract for 25 min, centrifuge at room temperature for 25 min at a speed of 3500 rpm, and concentrate the extract by a rotary evaporator until the solvent is exhausted; Add 400 μl deuterated methanol and 400 μl heavy water (containing NaH 2 PO 4 and 0.05% TSP, adjusted to pH=6.0 with NaOD for dissolution, the solution was centrifuged at 13,000 rpm for 10 min, and 600 μl of supernatant was pipetted into an NMR tube for NMR testing.

[0021] 2) Spectrum collection: The sample is mea...

Embodiment 2

[0026] Embodiment 2, the judgment of unknown sample

[0027] Select 10 batches of Astragalus samples in addition, carry out test and analysis according to the steps of Example 1, calculate the ratio of sucrose / (α-glucose+β-glucose) to judge, as shown in Table 2, 10 batches of samples are accurately discriminated, and the accuracy rate is 100%. .

[0028] Table 210 batch sample analysis and judgment results

[0029]

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Abstract

The invention provides a method for distinguishing wild radix astragali from cultured radix astragali. The method comprises the following steps of acquiring a nuclear magnetic resonance hydrogen spectrum of radix astragali crude medicine by a nuclear magnetism technology, respectively carrying out manual integration on sucrose, alpha-glucose and beta-glucose signals, calculating the integral area ratio of sucrose to glucose, and judging the growth pattern of the unknown radix astragali crude medicine according to the range of the ratio to accurately distinguish the wild radix astragali from the cultured radix astragali. The method can be used for identifying the quality of the radix astragali crude medicine.

Description

technical field [0001] The invention relates to a technique for identifying the quality of medicinal materials, in particular to a method for identifying wild Astragalus membranaceus and cultivated Astragalus membranaceus. Background technique [0002] Astragalus membranaceus is the dried root of Astragalus membranaceus (Fisch.) Bge. or Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao of the leguminous Astragalus plant. "Shen Nong's Materia Medica" lists it as the top grade. It has the functions of invigorating qi and raising yang, strengthening the surface and antiperspirant, diuresis and detumescence, promoting body fluid and nourishing blood. Cardiac insufficiency, hepatitis and other diseases caused by spleen deficiency and dampness. [0003] Astragalus is a perennial herb, which has traditionally been wild or imitated wild. It grows for at least 4 years and is usually harvested in 6 to 8 years. After the founding of the People's Republic of China, wit...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 李震宇李爱平张丽增秦雪梅
Owner SHANXI UNIV
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