Method for utilizing protein and sugar of tobacco leaf to prepare tobacco flavor

A technology for tobacco flavor and protein, which is applied to the preparation of tobacco, essential oils/spice, tobacco, etc., can solve problems such as complex enzymatic hydrolysis treatment process, and achieve the effects of simple process, comfortable aftertaste, and comfortable fragrance coordination

Inactive Publication Date: 2014-11-12
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the disadvantages of complex enzymatic treatment process and the need to add brown sugar as a reducing sugar source.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing tobacco essence by using tobacco leaf's own protein and sugar, comprising the following steps:

[0026] (1) Simultaneous extraction of tobacco leaf protein and sugar: dry and crush the tobacco leaves extracted by ethanol solution to 20 mesh tobacco leaf fragments, weigh 100g of the resulting tobacco leaf fragments and place them in an ultrasonic circulation extractor, add 1000mL of KOH with a pH value of 8 solution, at a temperature of 60°C and an ultrasonic power of 100W, ultrasonic circulation extraction for 30 minutes, and filtering to obtain a mixed extract containing protein, amino acid and sugar. The amino acid in the mixed extract is a small amount of free amino acid in the original tobacco leaves .

[0027] (2) Enzymatic hydrolysis of tobacco leaf protein: Concentrate the mixed extract to 500mL, add KHCO 3 The solution adjusted the pH value of the concentrated solution to 6.5, then added 0.2 g of complex protease, and reacted at 50°C for ...

Embodiment 2

[0030] A method for preparing tobacco essence by using tobacco leaf's own protein and sugar, comprising the following steps:

[0031] (1) Simultaneous extraction of tobacco leaf protein and sugar: dry and crush the tobacco leaves extracted with ethanol solution to 20 mesh tobacco leaf fragments, weigh 100g of the resulting tobacco leaf fragments and place them in an ultrasonic circulation extractor, add 1200mL of KOH with a pH value of 8 solution, at a temperature of 50°C and an ultrasonic power of 300W for 30 minutes of ultrasonic circulation extraction, filtered to obtain a mixed extract containing protein, amino acid and sugar, the amino acid in the mixed extract is a small amount of free amino acid in the original tobacco leaves .

[0032] (2) Enzymatic hydrolysis of tobacco leaf protein: Concentrate the mixed extract to 500mL, add KHCO 3 The solution adjusted the pH value of the concentrated solution to 6.5, then added 0.15 g of complex protease, and reacted at 50°C for ...

Embodiment 3

[0035] A method for preparing tobacco essence by using tobacco leaf's own protein and sugar, comprising the following steps:

[0036] (1) Simultaneous extraction of tobacco leaf protein and sugar: dry and pulverize the tobacco leaves extracted by ethanol solution to 20 mesh tobacco leaf fragments, weigh 150g of the resulting tobacco leaf fragments and place them in an ultrasonic circulation extractor, add 2250mL of KOH with a pH value of 9 solution, at a temperature of 60°C and an ultrasonic power of 300W for 60 minutes of ultrasonic circulation extraction, filtered to obtain a mixed extract containing protein, amino acid and sugar, the amino acid in the mixed extract is a small amount of free amino acid in the original tobacco leaves .

[0037] (2) Enzymatic hydrolysis of tobacco leaf protein: Concentrate the mixed extract to 800mL, add KHCO 3 The solution adjusted the pH value of the concentrated solution to 6.0, then added 0.3 g of complex protease, and reacted at 55°C for...

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Abstract

The invention discloses a method for utilizing protein and sugar of tobacco leaf to prepare tobacco flavor, which comprises the following steps: (1) drying and crushing the tobacco leaf, then putting into a circulating type ultrasonic extractor, adding an alkaline aqueous solution for performing ultrasonic circulation extraction, and filtering, thereby acquiring a mixed extraction solution containing protein, amino acid and sugar; (2) concentrating the mixed extraction solution, adjusting pH value of the acquired concentrated solution, adding compound protease and performing deep enzymolysis, and then concentrating again, thereby acquiring the mixed enzymatic hydrolysate rich in amino acid, small molecule peptide and sugar; (3) taking the amino acid, the small molecule peptide and the sugar in the mixed enzymatic hydrolysate as raw materials and performing maillard reaction, thereby acquiring the tobacco flavor. According to the invention, the protein and the sugar which are efficiently extracted from the tobacco leaf are utilized to prepare the tobacco flavor, no additional raw materials are added, and the prepared flavor can effectively reduce the irritation of tobacco and approaches the tobacco flavor.

Description

technical field [0001] The invention belongs to the technical field of deep processing of tobacco leaves, and in particular relates to a method for preparing tobacco essence by utilizing protein and sugar contained in tobacco leaves itself. Background technique [0002] Protein is of great significance in the growth process of tobacco, but for tobacco products, when the overall protein content is too high, it is an unfavorable chemical component. The protein content in tobacco leaves is 6% to 7%. When the protein content in tobacco leaves is too high, exceeding 15%, it will cause excessive smoke intensity, poor aroma and taste, and produce pungent and bitter taste, thereby affecting the quality of cigarettes. It is more difficult to directly extract tobacco protein as a kind of edible protein, because it is often combined with other substances in tobacco, difficult to purify, and has poor color and taste. During the production process of reconstituted tobacco leaves, most o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/30A24B15/18A24B3/12C11B9/02
Inventor 刘治田张旗郑乐驰
Owner WUHAN INSTITUTE OF TECHNOLOGY
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