Decalcified dental matrix of mammal and preparation method thereof

Abstract

The invention relates to a decalcified dental matrix of a mammal and a preparation method thereof. The raw material is from permanent teeth of the mammal. The preparation method comprises the following steps: (1) collecting permanent teeth of the mammal for later use; (2) soaking the permanent teeth in a 50% 84 disinfectant for sterilizing, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (3) removing useless parts of the teeth; (4) smashing tooth tissues into particles; (5) sterilizing the smashed tooth tissue particles, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (6) putting the treated tooth tissue particles into 0.65-0.72 equivalent weight of hydrochloric acid liquid for soaking for 10-16 hours at 34-38DEG C to finish decalcification; and (7) drying at low temperature, soaking in hydrogen peroxide for 30 minutes, so as to obtain the decalcified dental matrix of the mammal. The decalcified dental matrix of the mammal has better inductive bone regenerative action, and does not carry any pathogenic factor after being prepared into final products.

Description

technical field [0001] The invention relates to a medical material and a manufacturing method thereof, in particular to a decalcified mammalian tooth matrix and a manufacturing method thereof. Background technique [0002] A scholar in the United States put forward the theory of osteoinduction, thinking that in animals and human hard tissues (bone and teeth), there is a bone morphogenetic protein (BMP), which has the function of inducing bone regeneration in the body. Choose fresh, healthy animal or human hard tissue as raw material, remove the soft tissue through mechanical processing, crush it into granules, wash it with water and then go through chemical processing to remove fat, and then put the hard tissue fragments into 0.6 equivalent hydrochloric acid solution for constant temperature decalcification Prepare hard tissue matrix. After this kind of hard tissue matrix is ​​transplanted into the body, the bone morphogenetic protein contained in it can produce directional...

AI Technical Summary

Problems solved by technology

[0003] However, looking at the research process at home and abroad for more than 30 years, it is found that the reason why there is no major progress in the use of teeth as raw materials to make osteoinductive materials is because people have been making mistakes in choosing raw teeth and using deciduous teeth as raw materials by mistake. No valuable experimental results can be obtained