Method for Separating Microthrix Using Polydimethylsiloxane Hydrophobic Material Plate
A technology of polydimethylsiloxane and hydrophobic materials, applied in the field of environmental microorganisms, can solve the problems of complex process, high operation requirements, time-consuming, etc., shorten the pure culture period, overcome separation difficulties, and be simple and easy to operate Effect
Active Publication Date: 2016-08-17
TIANJIN CHENGJIAN UNIV
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Problems solved by technology
At present, the traditional separation and cultivation methods of Microthrix mainly include the dilution plate method and the coating plate method. Since the activated sludge contains a large number of bacteria that do not cause sludge bulking, this makes the process of using the traditional Microthrix isolation method complicated. Complicated, time-consuming and difficult to obtain pure strains and other disadvantages (vanVeen, 1973; Seviour et al., 1994; Blackall et al., 1994)
In addition, it has also been reported to adopt micromanipulation technology to realize the separation of Microthrix, such as Italy's Rossetti et al. (1997) adopted Skerman micromanipulation technology to isolate and cultivate the pure strain of Microthrix, but this technology requires high operation requirements. In addition, there is some uncertainty about whether the selected filamentous bacteria are microthrix
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example 1
[0021] The activated sludge mixed solution used in this example is the expanded sludge mixed solution in the aeration tank of a sewage treatment plant in Tianjin, with Microthrix as the dominant bacterium. Take 150 mL of the expanded sludge mixed solution and add it to a beaker, and put Perform strong magnetic stirring in a water bath for 2 hours, and use a pipette to quantitatively take out 100 μL of the stirred sludge mixture and drop it on the selected material plate, and store it in the refrigerator for 1 hour. After taking it out, use a concentration of 10 mmol / L The sodium chloride solution was used to rinse it 3 times, and the dosage of each sodium chloride solution was 2mL, and then the adhesion of microthrix on the surface of the material plate was observed under a microscope.
[0022] In order to illustrate the effect of the hydrophobicity and groove structure of the material plate involved in the present invention on the separation effect of microthrix, a glass plate...
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Abstract
The invention provides a method for isolating microthrix by using a polydimethylsiloxane hydrophobic material plate. The step of the method is to mix the swelling sludge in the aeration tank of the sewage treatment plant with microthrix as the dominant bacteria Droplets are added to the polydimethylsiloxane hydrophobic material plate with a groove structure, and after being refrigerated and stored, the polydimethylsiloxane hydrophobic material plate is rinsed with sodium chloride solution, and the principle of similar compatibility is used. Realize the separation of microthrix from the activated sludge mixture. The effect of the present invention is that the method is simple and easy to operate, can quickly realize the separation of microthrix from the activated sludge mixed solution, overcomes the problem of difficult separation of microthrix from the activated sludge system, and can separate microthrix The polydimethylsiloxane hydrophobic plate of the mycelium is placed in the culture medium to carry out the pure culture of the microthrix, which is faster than the traditional methods of pure culture of the microthrix such as the dilution plate method and the coating plate method. The screening of microthrix strains can shorten the period of pure culture of microthrix by 3 to 6 weeks.
Description
technical field [0001] The invention belongs to the field of environmental microorganisms, and mainly relates to a method for separating microthrix by using a polydimethylsiloxane (PDMS) hydrophobic material plate. Background technique [0002] Activated sludge method is currently the most widely used urban sewage treatment technology in the world, but the sludge bulking problem in its operation process has always been a common problem affecting the normal operation of sewage treatment plants. Its occurrence rate is high and it is harmful Big and difficult to manage. The study found that most of the sludge bulking in sewage treatment plants is filamentous bacteria sludge bulking, which is mainly caused by the reproduction of a large number of filamentous bacteria. Filamentous bacteria protrude a long mycelium from the sludge floc, and the bacteria The filaments are in contact with each other, forming a frame structure through bridging, supporting the sludge flocs, making it...
Claims
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IPC IPC(8): C12N1/20C12R1/01
Inventor 费学宁李松亚曹凌云闫卫国焦秀梅
Owner TIANJIN CHENGJIAN UNIV