Establishment method of breast milk fatty acid profile database

Abstract

An establishing method of a breast milk fatty acid spectrum database is disclosed. The method includes following steps of: preparing a plurality of breast milk samples, subjecting each of the samples to esterification with methanol, centrifuging reaction liquids after the reactions are finished to obtain the upper organic phases, injecting the organic phases to SPE solid phase extraction cartridges, eluting the SPE solid phase extraction cartridges with an eluant, collecting the eluates, performing blown-drying with N2 to obtain purified fatty acid methyl esters, collecting fatty acid methyl ester spectrums by utilization of gas chromatography, calculating the content of each fatty acid in each of the samples by utilization of a normalization method, and establishing the breast milk fatty acid spectrum database. A breast milk fatty acid measuring method can rapidly and accurately measure the contents of the fatty acids in the breast milk, and changing characteristics of variety and contents of breast milk fatty acids of different populations during the lactation period can be knew, thus establishing the breast milk fatty acid spectrum database and guiding enterprises in china to produce formula milk powder truly suitable for China infants.

Description

technical field [0001] The invention relates to the field of fatty acid content determination, in particular to a method for establishing a breast milk fatty acid spectrum database. Background technique [0002] Breast milk is the only most natural, safest and most complete natural food for infant growth. It is rich in nutrients and contains carbohydrates, proteins, fats, vitamins, minerals, antibodies and other substances needed for infant growth. It is easy to absorb and utilize, and can ensure healthy growth of the baby. In particular, 50% of the fat contained in breast milk can not only provide the daily calories consumed by the baby's body, but also meet the fat needed for the baby's brain development. Breast milk can provide essential fatty acids (essentialfattyacid, EFA), such as linoleic acid (LA) and α-linolenic acid (linoleic acid, LNA) and some long chain polyunsaturated fatty acids (longchainpolyunsaturatedfattyacid, LCPUFA), which cannot be synthesized in the b...

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Problems solved by technology

[0005] In the Chinese population, there is no rapid and accurate method for establishing a breast milk fatty acid profile database. The establishment of this method is useful for building a breast milk fatty acid profile database for populations in different regions, understanding the characteristics of breast milk in different regions of China and the changes in breast milk fatty acids during lactation It is of great significance to guide Chinese enterprises to produce real formula milk powder suitable for Chinese infants based on the breast milk fatty acid profile database

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