siRNA molecular composition and application thereof in treatment on pathological scars

A composition, a technology for hypertrophic scar, applied in the fields of molecular biology and gene therapy to achieve the effect of promoting apoptosis of fibroblasts

Inactive Publication Date: 2014-12-03
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this document discloses that the application of siRNA to wounds can promote healing and reduce scar formation during the healing process. Wound treatment and scar treatment are two completely different concepts, involving different physiological processes. There is no report on the treatment of hypertrophic scars and keloids that have formed

Method used

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  • siRNA molecular composition and application thereof in treatment on pathological scars
  • siRNA molecular composition and application thereof in treatment on pathological scars
  • siRNA molecular composition and application thereof in treatment on pathological scars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1 Experimental materials

[0039] 1.1 Active ingredients of siRNA

[0040] TGF-β1 siRNA molecule: 5′-CCCAAGGGCUACCAUGCCAACUUCU-3′ (SEQ ID NO.1);

[0041]COX-2 siRNA molecule: 5'-GGUCUGGUGCCUGGUCUGAUGAUGU-3' (SEQ ID NO.2);

[0042] Drug carrier: peptide (HKP), a polymer of histidine and lysine;

[0043] STP705 (siRNA cocktail): the above-mentioned TGF-β1 siRNA molecule, COX-2 siRNA molecule and HKP are formed into nanoparticles, wherein the copy number ratio of TGF-β1 siRNA molecule and COX-2 siRNA molecule is 1:1, and the weight ratio of the two siRNA molecules to HKP It is 1:4. The above drugs were provided by Suzhou Shengnuo Biological Co., Ltd.

[0044] Antibody

[0045] Rabbit anti-human α-actin (α-SMA, alphasmoothmuscleActin) monoclonal antibody (ab5694, Abcam, Iowa, USA), the dilution ratio of the primary antibody for immunohistochemical staining is 1:200, and the dilution ratio of the primary antibody for immunofluorescence staining is 1:100 ;

[0046] Rab...

Embodiment 2

[0125]Compare the siRNA molecular composition of the present invention with the siRNA molecular composition in Chinese patent document CN201310577630.0 (siRNA molecular composition, the siRNA molecular composition comprises: the nucleotide sequence contains a) 5'-ccccggaggugauuuccaucuacaa-3' , 5'-uuguagauggaaaucaccuccgggg-3' siRNA molecule, and nucleotide sequence containing b) 5'-gucuuuggucuggugccuggucuga-3', 5'-ucagaccaggcaccagaccaaagac-3' siRNA molecule) in regression hypertrophic scar or keloid .

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Abstract

The invention relates to a siRNA molecular composition and application thereof in treatment on hypertrophic scars and keloids. The siRNA molecular composition contains siRNA molecules with the sequence of 5'-CCCAAGGGCUACCAUGCCAACUUCU-3' and siRNA molecules with the sequence of 5'-GGUCUGGUGCCUGGUCUGAUGAUGU-3'. The siRNA molecular composition is proved to be capable of promoting in-vitro fibroblast apoptosis of the hypertrophic scars and keloids and have a good treatment effect on the hypertrophic scars in an animal model.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and gene therapy, in particular to the application of siRNA molecules in the treatment of hypertrophic scars and keloids. Background technique [0002] The skin is the largest organ of the human body and has functions such as protection, immunity, and excretion, among which the most important function is to protect and maintain the homeostasis of the internal environment. When the integrity of the skin is greatly damaged, the homeostasis in the body becomes unbalanced, and external invasion factors easily enter the body, directly threatening the survival of the individual. In the process of species evolution, the scar healing mode of wounds appeared. This healing method can restore the integrity of the skin as quickly as possible, greatly improving the survival rate of the species. Therefore, scar is an indispensable product of wound healing in human skin. [0003] However, in the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P17/02
Inventor 周佳刘凯黄晓璐李青峰
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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