Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

siRNA molecular composition and application thereof in treatment on pathological scars

A composition, a technology for hypertrophic scar, applied in the fields of molecular biology and gene therapy to achieve the effect of promoting apoptosis of fibroblasts

Inactive Publication Date: 2014-12-03
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this document discloses that the application of siRNA to wounds can promote healing and reduce scar formation during the healing process. Wound treatment and scar treatment are two completely different concepts, involving different physiological processes. There is no report on the treatment of hypertrophic scars and keloids that have formed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • siRNA molecular composition and application thereof in treatment on pathological scars
  • siRNA molecular composition and application thereof in treatment on pathological scars
  • siRNA molecular composition and application thereof in treatment on pathological scars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1 Experimental materials

[0039] 1.1 Active ingredients of siRNA

[0040] TGF-β1 siRNA molecule: 5′-CCCAAGGGCUACCAUGCCAACUUCU-3′ (SEQ ID NO.1);

[0041]COX-2 siRNA molecule: 5'-GGUCUGGUGCCUGGUCUGAUGAUGU-3' (SEQ ID NO.2);

[0042] Drug carrier: peptide (HKP), a polymer of histidine and lysine;

[0043] STP705 (siRNA cocktail): the above-mentioned TGF-β1 siRNA molecule, COX-2 siRNA molecule and HKP are formed into nanoparticles, wherein the copy number ratio of TGF-β1 siRNA molecule and COX-2 siRNA molecule is 1:1, and the weight ratio of the two siRNA molecules to HKP It is 1:4. The above drugs were provided by Suzhou Shengnuo Biological Co., Ltd.

[0044] Antibody

[0045] Rabbit anti-human α-actin (α-SMA, alphasmoothmuscleActin) monoclonal antibody (ab5694, Abcam, Iowa, USA), the dilution ratio of the primary antibody for immunohistochemical staining is 1:200, and the dilution ratio of the primary antibody for immunofluorescence staining is 1:100 ;

[0046] Rab...

Embodiment 2

[0125]Compare the siRNA molecular composition of the present invention with the siRNA molecular composition in Chinese patent document CN201310577630.0 (siRNA molecular composition, the siRNA molecular composition comprises: the nucleotide sequence contains a) 5'-ccccggaggugauuuccaucuacaa-3' , 5'-uuguagauggaaaucaccuccgggg-3' siRNA molecule, and nucleotide sequence containing b) 5'-gucuuuggucuggugccuggucuga-3', 5'-ucagaccaggcaccagaccaaagac-3' siRNA molecule) in regression hypertrophic scar or keloid .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a siRNA molecular composition and application thereof in treatment on hypertrophic scars and keloids. The siRNA molecular composition contains siRNA molecules with the sequence of 5'-CCCAAGGGCUACCAUGCCAACUUCU-3' and siRNA molecules with the sequence of 5'-GGUCUGGUGCCUGGUCUGAUGAUGU-3'. The siRNA molecular composition is proved to be capable of promoting in-vitro fibroblast apoptosis of the hypertrophic scars and keloids and have a good treatment effect on the hypertrophic scars in an animal model.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and gene therapy, in particular to the application of siRNA molecules in the treatment of hypertrophic scars and keloids. Background technique [0002] The skin is the largest organ of the human body and has functions such as protection, immunity, and excretion, among which the most important function is to protect and maintain the homeostasis of the internal environment. When the integrity of the skin is greatly damaged, the homeostasis in the body becomes unbalanced, and external invasion factors easily enter the body, directly threatening the survival of the individual. In the process of species evolution, the scar healing mode of wounds appeared. This healing method can restore the integrity of the skin as quickly as possible, greatly improving the survival rate of the species. Therefore, scar is an indispensable product of wound healing in human skin. [0003] However, in the pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P17/02
Inventor 周佳刘凯黄晓璐李青峰
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com