Multimodal microscopic imaging system

Abstract

A multimodal microscopic imaging system performs detection by an optical means. Lasers generated by a laser source enter a light filter through a light beam collimation and beam expanding mechanism to be filtered, then, part of the lasers enter a reference arm to generate weak coherent signals through a spectroscope, the other part of the lasers sequentially pass through a collimation coupling mechanism and a light beam guiding and focusing mechanism to form focused light beams, the focused light beams enter an electric scanning device arranged on a detection window through an ultrasonic transducer, biological tissue to be detected is subjected to circular scanning through the electric scanning device, and the focused light beams induce the biological tissue to generate back scattering photons and opto-acoustic signals on the biological tissue to be detected. The internal structure and the functions of the biological tissue are imaged, and fast multimodal two-dimensional and three-dimensional images are provided for accurately monitoring the internal structure and function changes of the biological tissue.

Description

technical field [0001] The invention relates to a detection system using optical means, in particular to a multi-mode microscopic imaging system. Background technique [0002] Ultrasonic endoscopic imaging (USE) is the most commonly used imaging technology in the field of clinical biomedicine. It is mainly based on the detection of the mechanical properties of biological tissues and the differences in mechanical properties of biological tissues. interface imaging. Optical coherence tomography (OCT) mainly uses the weakly coherent interference signals of tissue scattered photons to detect the changes in the back reflection or scattering intensity of incident photons by tissues at different depths inside biological tissues, so as to obtain biological data within a certain depth range. Tissue microstructure information, and then obtain two-dimensional or three-dimensional structure imaging of biological tissue through transverse scanning. Photoacoustic microscopic imaging tec...

AI Technical Summary

Problems solved by technology

However, the optical system of this technology uses a dual light source system. When adjusting the optical path, it is difficult to ensure that the two beams of light are completely coaxial when passing through the dichroic mirror through the adjustment of the optical device, and at the same time increase the cost of the system and the cost of the system. Complexity; and the scanning method is mechanical movement, the scanning speed is limited by the huge mechanical device, and the measurement accuracy is also limited; at the same time, due to the movement of the sample driven by the machine, the sample needs to have very good stability, and the scanning area matching accuracy is limited ; and it is mainly a combination of three separate imaging systems, which cannot achieve endoscopic multi-modal microscopic imaging

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