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A strain of Bacillus subtilis 2012syx04 for controlling rice blast

A technology of 2012SYX04 and Bacillus subtilis, applied in the field of microorganisms, to achieve high control efficiency, good control effect, and broad-spectrum control effect

Active Publication Date: 2016-05-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and application of Bacillus subtilis to control rice blast is the need for sustainable development of rice production, and can meet the requirements of the United Nations World Food Organization for the development of food security. Bacillus subtilis strain with high production of antibacterial active substances

Method used

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  • A strain of Bacillus subtilis 2012syx04 for controlling rice blast
  • A strain of Bacillus subtilis 2012syx04 for controlling rice blast
  • A strain of Bacillus subtilis 2012syx04 for controlling rice blast

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Screening and fermentation of Bacillus subtilis 2012SYX04 strain

[0028] (1) Sample collection: The samples were collected from the leaves of late-maturing rice varieties at Ciyun Town, Jiangjin District, Chongqing City. Pull out together with the root system of rice, pack it in a polyethylene plastic bag, and bring it back to the laboratory for isolation.

[0029] (2) Isolation of Bacillus subtilis: cut the healthy leaves of rice and put them in a 75% alcohol triangular flask, shake and wash them for 2 times, pour off the alcohol, add sterile water to soak, then draw the diluted solution and apply it on the NA medium plate. After drying, they were incubated upside down in a constant temperature incubator at 30°C for 48 hours. A single colony was picked, and the picked single colony was purified by gradient streak separation, and the purified strain was stored in NA slant medium for future use.

[0030] Broth medium (NA): peptone 10g, beef powder 3g, sodiu...

Embodiment 2

[0036] Embodiment 2: The biological activity assay of Bacillus subtilis 2012SYX04 bacterial strain:

[0037] (1) Determination of the inhibition of Bacillus subtilis 2012SYX04 strain on Magnaporthe grisea P131: Place the rice blast fungus cake in the center of the tomato oat plate medium, and inoculate the prepared 2012SYX04 at a distance of 2cm from both sides of the cake with a toothpick. The rice blast fungus cake was used for confrontation culture, and sterile water was used as a control, and it was placed in an incubator at 28°C for dark culture, and each treatment was repeated 3 times. After 12 days, the diameter of the pathogenic bacteria colony and the zone of inhibition were measured, and the results are shown in Table 1.

[0038] (2) Antibacterial determination of Bacillus subtilis 2012SYX04 fermentation culture liquid on rice blast fungus: place blast fungus fungus cake in the center of the tomato oat medium plate, use a puncher to punch holes at a distance of 2 cm fr...

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Abstract

The invention discloses a bacillus subtilis 2012SYX04 for preventing and treating rice blast and an application thereof. The bacillus subtilis has a broad-spectrum and high-efficient preventing effect on the rice blast and can be used for preventing and treating the rice blast on various strains of rice, especially for preventing and treating leaf blast and panicle blast.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Bacillus subtilis for controlling rice blast and its application. Background technique [0002] Rice is an important food crop in the world. Its output accounts for more than 1 / 4 of the world's total food production, more than half of China's total food production, and is the staple food that more than half of the world's population depends on. Rice blast (RiceBlast) is a kind of sudden and strong, easy to epidemic worldwide fungal disease caused by Ascomycete Magnaportheoryzae (T.T.Hebert) Yaegashi & Udagawa (asexual state: Pyriculariaoryzae). The disease can cause harm to rice all year round, and its harm spreads all over the various parts of rice, including seedling rice blast, leaf blast, leaf pillow blast, node rice blast, ear neck blast, branch blast and grain blast. Rice blast is widely distributed and occurs in 85 countries around the world. It is also one of the most...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 王琦沙月霞李燕付学池
Owner CHINA AGRI UNIV