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Quick determination method of oligosaccharides in adulterated honey

A detection method and rapid technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of high sensitivity, high selectivity and simple operation

Active Publication Date: 2014-12-17
JIANGSU SINOGRAPHY TESTING
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Problems solved by technology

[0003] The purpose of the present invention is to propose a fast qualitative and quantitative detection method for oligosaccharides in adulterated honey with good selectivity, high sensitivity and simple operation for the adulterated honeys currently on the market

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  • Quick determination method of oligosaccharides in adulterated honey
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Embodiment Construction

[0017] 1. Pretreatment: Accurately weigh 1.0 (±0.01) g of sample in a 50 ml sample tube, add 5.0 ml of water, vortex until completely dissolved, take 200 μl into the sample bottle, then add 800 μl of 7:3 acetonitrile: water to dilute to 1ml, vortex to mix, pass through the aqueous phase membrane and inject the sample.

[0018] 2. Preparation of standard solutions of pentasaccharides, hexasaccharides and heptasaccharides: Accurately weigh 0.025g of standard pentasaccharides, hexasaccharides and heptasaccharides respectively, and set the volume in a 25mL volumetric flask with ultrapure water to obtain 1mg / ml Standard solution, and then diluted to 1μg / ml standard solution. Finally, six standard points were obtained by dilution: 5ppb, 10ppb, 20ppb, 50ppb, 100ppb, and 200ppb, and the standard curves of pentasaccharide, hexasaccharide, and heptasaccharide were respectively obtained.

[0019] 3. Quantification by external standard method, the standard curve equation of pentasacchari...

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Abstract

The invention belongs to the technical field of food detection, in particular relates to a quick determination method of oligosaccharides in adulterated honey, and aims to develop a quickly and accurate method for qualitative and quantitative detection of the oligosaccharides in syrup in allusion to a part of honey adulterated with the syrup in the market in the prior art. The invention discloses the quick determination method of the oligosaccharides in the adulterated honey so as to achieve the purpose, and the quick determination method comprises three steps of pretreatment, liquid chromatographic separation and mass spectrometric detection. According to the HPLC-MS detection method, ion peaks with the mass to charge ratio of 851.3, 1013.5 and 1175.5m / z can be respectively used as feature detection peaks of pentasaccharides, hexaoses and heptaoses.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to a rapid detection method for oligosaccharides in adulterated honey. Background technique [0002] Pure natural honey is mainly composed of monosaccharides, including glucose and fructose, and also contains a small amount of disaccharides and trisaccharides. Real honey is brewed in the hive from the nectar collected by insect bees from the flowers of flowering plants. During the process from brewing to collecting honey, any artificial addition of active ingredients or pollen in honey is prohibited. However, in order to gain higher profits, some unscrupulous businessmen often add invert syrup similar to the main component of honey, such as beet syrup, rice syrup, tapioca syrup, and chicory syrup, to honey. In the past, there were carbon isotope methods (C4 plant sugars), thin-layer chromatography (TLC), fructofuranosidase method (BFF method), and European isotope method (LC...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 徐锦忠吴斌张晓燕刘秀红丁涛张睿杨波
Owner JIANGSU SINOGRAPHY TESTING