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Magnetic bead-based sample treatment and nucleic acid automatic extraction system

A technology for automatic extraction and nucleic acid extraction, which is applied in the fields of biomass post-processing, biological material pre-treatment, and stress-stimulated microbial growth methods. Effect

Inactive Publication Date: 2014-12-24
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process of sample processing and nucleic acid extraction includes several complex biological reaction steps, which often need to be done manually, the detection efficiency is low, and error-prone

Method used

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  • Magnetic bead-based sample treatment and nucleic acid automatic extraction system
  • Magnetic bead-based sample treatment and nucleic acid automatic extraction system

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Effect test

Embodiment 1

[0055] An automatic nucleic acid extraction system based on the principle of magnetic bead adsorption, which can automatically complete the operations of cell lysis, nucleic acid purification, and nucleic acid elution, and finally obtain a nucleic acid sample template suitable for gene amplification reactions. The principle of the biological reaction is as follows: the virus particles in the reaction sample are cleaved by the lysate, and the nucleic acid released after the cell lysis is absorbed by the magnetic beads, and then various kinds of washing liquid are used to remove all kinds of biological impurities in the sample, such as cell walls, etc. Proteinoid removal, nucleic acid purification, and finally, the nucleic acid is separated from the magnetic beads with an eluent to obtain a high-purity nucleic acid template. An inverted U-shaped connector-type dam channel is designed in the chip to connect the reaction chamber with the waste liquid chamber. Based on the siphon pr...

Embodiment 2

[0060] An automatic nucleic acid extraction system based on the principle of magnetic bead adsorption, which can automatically complete the operations of cell lysis, nucleic acid purification, and nucleic acid elution, and finally obtain a nucleic acid sample template suitable for gene amplification reactions. In a fully automatic and closed sampling working mode, a reaction reagent storage module is integrated on the microfluidic chip. During the reaction process, each reaction reagent is gradually injected into the reaction chamber according to the operation steps of the nucleic acid extraction reaction. Fully automated nucleic acid extraction with integrated microfluidic chips. The principle of the biological reaction is as follows: the virus particles in the reaction sample are cleaved by the lysate, and the nucleic acid released after the cell lysis is absorbed by the magnetic beads, and then various kinds of washing liquid are used to remove all kinds of biological impuri...

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Abstract

The invention discloses a magnetic bead-based sample treatment and nucleic acid automatic extraction system, comprising a micro-fluidic chip and a micro motor drive device, wherein the micro-fluidic chip comprises a layer of cover plate, two layers of bottom plates and two layers of structure plates, wherein the chip can automatically finish the operation processes such as cell lysis, nucleic acid purification, nucleic acid eluting and the like, and finally a nucleic acid sample is obtained. Based on siphon principle or micro-fluidic surface tension properties, a driven drive pump without external drive force is designed by the chip, so as to achieve flowing and transferring of a detection sample, a reaction reagent and reaction liquid waste. Under mixed hardening of a micro vibration motor, through a periodic alternating magnetic field and a constant magnetic field provided by an external magnet, effective mixing between the magnetic bead and the reaction sample and the reaction reagent in a reaction cavity, and fixing of the magnetic bead in the liquid waste discharge process are achieved; and the chip is matched with a full-automatic micro motor drive device to automatically finish a plurality of reaction steps, so that a high-purity nucleic acid sample template is provided for gene amplification reaction.

Description

technical field [0001] The invention relates to the technical field of microfluidic biochips, which can automatically complete the process of sample processing and nucleic acid extraction. Based on the adsorption of magnetic beads to nucleic acid, the invention automatically completes multiple reaction steps such as cell lysis, nucleic acid purification, and nucleic acid elution through the cooperation between the microfluidic chip and its supporting driving device, and obtains a reaction suitable for gene amplification. nucleic acid template. Based on the siphon principle, or the surface tension characteristics of microfluidics, the chip designs a driven pump that does not require external driving force, and realizes the flow and transfer of detection samples, reaction reagents, and reaction waste liquid. Through the periodic motion control of the external magnet by the driving device, a periodic external alternating magnetic field is generated and provided, and the driving ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/42C12M1/36
CPCC12N15/1013
Inventor 邱宪波周轩葛胜祥张师音夏宁邵
Owner BEIJING UNIV OF CHEM TECH
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