A kit for detecting microdeletion of human y chromosome

A Y chromosome and kit technology is applied in the field of kits for detecting human Y chromosome microdeletions, which can solve the problems of unfavorable promotion, high professional requirements for result interpretation, expensive sequencers and HPLC, and avoid false negatives. , the effect of reducing the risk of pollution and reducing the workload
CN104232779BActive Publication Date: 2016-06-01亚能生物技术(深圳)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
亚能生物技术(深圳)有限公司
Publication Date
2016-06-01

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Abstract

The invention relates to the field of chromosome deletion, in particular to a kit for detecting the microdeletion of a human Y chromosome. The kit comprises PCR (Polymerase Chain Reaction) reaction liquid I, PCR reaction liquid II and PCR reaction liquid III, wherein the PCR reaction liquid I comprises primers and fluorescent probes of specific amplification ZFX / Y, sY254, sY134 and SRY (Sex-determine Region of Y Chromosome) sites; the PCR reaction liquid II comprises primers and fluorescent probes of specific amplification ZFX / Y, sY84 and sY127 sites; the PCR reaction liquid III comprises primers and fluorescent probes of specific amplification ZFX / Y, sY255 and sY86 sites. According to the kit disclosed by the invention, by designing the specific fluorescent probes of different STS (Sequence Tagged Site) and three-pipe reaction systems are optimally combined, so that microdeletion types of the Y chromosome can be detected by one-time test; effective monitoring for different reaction systems is realized by the ZFX / Y.
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Description

technical field

[0001] The invention relates to the field of chromosome deletion detection, in particular to a kit for detecting human Y chromosome microdeletion. Background technique

[0002] Azoospermiafactor (AZF), which is closely related to spermatogenesis, exists on the long arm of the human Y chromosome. The small deletion of DNA fragments in the azoospermia factor is called Y chromosome microdeletion. The occurrence of Y chromosome microdeletion can cause spermatogenesis disorder, resulting in oligozoospermia, weak sperm, teratozoospermia or even azoospermia. According to WHO statistics, about 10%-15% of male patients with primary azoospermia and oligospermia have Y chromosome microdeletions. In male infertility patients caused by spermatogenesis disorder, the incidence of Y chromosome microdeletion is the second genetic factor.

[0003] At present, AZF is divided into 3 regions, namely AZFa, AZFb and AZFc. Any one or more deletions in these regions will lead to sp...

Claims

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