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Method for measuring content of biomacromolecules of microalgae by adopting Fourier transform infrared method

A kind of microalgae biology, Fourier technology, applied in the direction of measurement device, analysis material, material analysis by optical means, etc., can solve the problems of large toxic side effects, interference, time-consuming

Active Publication Date: 2014-12-24
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the weighing method takes a long time, requires a large number of samples, and the Nile red fluorescent staining method has serious side effects and serious interference, and can only measure the oil content of microalgae, etc.

Method used

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  • Method for measuring content of biomacromolecules of microalgae by adopting Fourier transform infrared method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Measure the fat content of the Chlorella vulgaris (Chlorella vulgaris) that has been purely cultivated with detection method of the present invention, and concrete operating steps are as follows:

[0033] 1) Take Chlorella vulgaris as the object, first activate the algae species on BG-11 solid medium, pick a single clone and inoculate it into 20ml BG-11 liquid medium, 150 rpm, 1500-2500Lux, culture at 28°C for 48-72 hours , Transfer 20ml of the cultured algae liquid to 100ml of liquid medium, 28°C, 1500-2500Lux, static culture for 48-72 hours, when the OD reaches 1.0, the algae liquid is collected by centrifugation and prepared for FT-IR analysis.

[0034] 2) Concentrate the algae liquid: Centrifuge the above 100ml algae liquid at 6000rpm for 5 minutes, remove the supernatant, concentrate 100 times, and add 1ml of BG-11 liquid medium.

[0035] 3) Add anti-interference agent: In order to reduce the influence of water on infrared absorption, glycerol is added to make the ...

Embodiment 2

[0041] Measure the protein content of the Chlorella vulgaris (Chlorella vulgaris) that has been purely cultivated with detection method of the present invention, and concrete operating steps are as follows:

[0042] 1) Take Chlorella vulgaris as the object, first activate the algae species on BG-11 solid medium, pick a single clone and inoculate it into 20ml BG-11 liquid medium, 150 rpm, 1500-2500Lux, and culture at 28°C for 48-72 hours , Transfer 20ml of the cultured algae liquid to 100ml of liquid medium, 28°C, 1500-2500Lux, static culture for 48-72 hours, when the OD reaches 1.0, the algae liquid is collected by centrifugation and prepared for FT-IR analysis.

[0043] 2) Concentrate the algae liquid: Centrifuge the above 100ml algae liquid at 6000rpm for 5 minutes, remove the supernatant, concentrate 100 times, and add 1ml of BG-11 liquid medium.

[0044] 3) Add anti-interference agent: In order to reduce the influence of water on infrared absorption, glycerol is added to m...

Embodiment 3

[0050] Measure the carbohydrate content of the Chlorella vulgaris (Chlorella vulgaris) that has been purely cultivated with detection method of the present invention, concrete operation steps are as follows:

[0051] 1) Take Chlorella vulgaris as the object, first activate the algae species on BG-11 solid medium, pick a single clone and inoculate it into 20ml BG-11 liquid medium, 150 rpm, 1500-2500Lux, and culture at 28°C for 48-72 hours , Transfer 20ml of the cultured algae liquid to 100ml of liquid medium, 28°C, 1500-2500Lux, static culture for 48-72 hours, when the OD reaches 1.0, the algae liquid is collected by centrifugation and prepared for FT-IR analysis.

[0052] 2) Concentrate the algae liquid: Centrifuge the above 100ml algae liquid at 6000rpm for 5 minutes, remove the supernatant, concentrate 100 times, and add 1ml of BG-11 liquid medium.

[0053] 3) Add anti-interference agent: In order to reduce the influence of water on infrared absorption, glycerol is added to ...

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Abstract

The invention relates to a method for measuring the content of biomacromolecules of microalgae by adopting a Fourier infrared method. The method comprises the steps of collecting purely cultured algae strains from a natural water body, culturing the algae strains until the OD680 reaches 1.0, centrifugating an algae liquid, removing supernate, concentrating and adding glycerol; performing 1,000-3,000cm<-1> continuous detection analysis on the sample to be detected by an FTIR ReactIRTM45m Fourier transform infrared spectrometer; detecting a local bottom by FT-IR; performing statistical analysis on a result by software iC QuantTM so as to obtain the actual content of the biomacromolecules; and substituting a measurement result into a formula to calculate the contents TL (mg), TP (mg) and TC (mg) of the biomacromolecules of oil AL, proteins Ap and carbon benzene hydrates Ac. The method is harmless to cells and can quickly detect the content of oil of the microalgae and the contents of proteins and carbohydrates in the microalgae.

Description

technical field [0001] The invention belongs to the technical field of microalgae bioengineering. In particular, it relates to a method for measuring oil content, protein and carbohydrate of microalgae, especially for non-invasive rapid screening, detection and / or separation of microalgae cells in growth culture. Background technique [0002] Microalgae are a type of plankton with extremely small size (0.2-500 μm) and huge numbers, which are ubiquitous in the oceans covering 71% of the earth's area. They are the primary producers in the marine ecosystem, and they produce all other heterotrophic organisms in the ocean. organic matter for survival. Microalgae contain chlorophyll a and are capable of photosynthesis. Due to its small size, large specific surface area, high metabolic activity, fast growth and reproduction, short doubling time, and easy cultivation, a large number of algae can be obtained; and its biodiversity is extremely high. The total number of species of aq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563
Inventor 孙洪磊吕静何皓傅鹏程齐泮仑兰玲付兴国胡徐腾
Owner PETROCHINA CO LTD