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Stabilizing composition for biological materials

A composition, carbohydrate technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems such as protein and enzyme cell rupture

Pending Publication Date: 2014-12-24
ADVANCED BIONUTRITION CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The freezing step of this method can have adverse effects such as denaturation of proteins and enzymes and rupture of cells

Method used

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  • Stabilizing composition for biological materials
  • Stabilizing composition for biological materials
  • Stabilizing composition for biological materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Preparation of dry and stable compositions

[0105] Basic Carb Mix

[0106] About 70 g of trehalose (Cargill Minneapolis, MN), about 5 g of instant inulin (Cargill Minneapolis, MN), and about 3 g of sodium alginate (ISP Corp., Wayne, NJ) were uniformly mixed in dry form.

[0107] Base Glass Reinforcing Mixture

[0108] About 17 g of casein hydrolyzate or pea hydrolyzate (ultrafiltered hydrolyzate, Marcor, Carlstadt, NJ) and 5 g of sodium citrate or sodium ascorbate (Sigma, St. Louis, MO) were uniformly mixed in dry form.

[0109] Stabilization of Probiotics

[0110] A fresh concentrate of L. rhamnosus (100ml, 10% solids, directly from the fermentation harvest) was added to the mixer and kept at 35°C. About 78 g of the base carbohydrate mix and about 22 g of the base glass enhancer mix were slowly added to the probiotic culture and mixed for 10 minutes at 35°C. The viscous slurry was then transferred to a container with a porous bottom and allowed to drip into a tank...

Embodiment 2

[0114] Storage Stability of Dried Probiotics

[0115] figure 1 The dry stable probiotics of Example 1 and commercially available dry probiotics (Culturelle, Amerifit, Inc., Cromwell, CT) were shown in two different accelerated storage conditions (40° C. and 33% RH and 30 Storage stability under ℃ and 43% RH). Commercially available probiotics completely lost their viability within the first few weeks under accelerated storage conditions, while the dry composition of the probiotics of the present invention lost only 1.18 log after 60 days at 30°C and 43% RH, at Only 1.09 log loss at 40°C and 33% RH.

Embodiment 3

[0117] Scaled-up production of a stable dry composition containing the probiotic Lactobacillus rhamnosus

[0118] Lactobacillus rhamnosus (400 g of frozen concentrate from a commercial source) was thawed at 37°C in a jacketed dual planetary mixer (DPM, 1qt, Ross Engineering, Inc. Savannah, GA) and the solids content was adjusted with distilled water. Adjust to 10% solids by weight. About 212 g of trehalose (Cargill Minneapolis, MN), about 20 g of instant inulin (Cargill Minneapolis, MN), about 12 g of sodium alginate (ISP Corp., Wayne, NJ), about 136 g of casein hydrolyzate ( Ultrafiltered hydrolyzate, Marcor, Carlstadt, NJ) and approximately 20 g of sodium ascorbate (Sigma, St. Louis, MO) were uniformly mixed in dry form. The powder mixture was slowly added to the probiotic culture and mixed for 10 minutes at 40 RPM, 37°C. The slurry was then transferred to a container with a porous bottom and allowed to drop into a tank containing liquid nitrogen. The beads were then remo...

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Abstract

Dry stabilizing compositions for bioactive materials include sugars and hydrolyzed proteins, and may be formed into tablets or other forms providing enhanced stability for the bioactive material. Compositions containing the bioactive materials may be produced by a method that includes (a) combining the bioactive material with other ingredients in an aqueous solvent to form a viscous slurry; (b) snap-freezing the slurry in liquid nitrogen to form solid frozen particles, beads, droplets or strings; (c) primary drying by water removal under vacuum of the product of step (b) while maintaining it at a temperature above its freezing temperature; and (d) secondary drying of the product of step (c) at maximum vacuum and a temperature of 20 DEG C or higher for a time sufficient to reduce the water activity to below 0.3 Aw.

Description

[0001] Cross References to Related Applications [0002] This application is a continuation-in-part of U.S. Application No. 13 / 378,106, filed March 29, 2012, which is a national phase entry of International Application No. PCT / US11 / 22821, filed January 28, 2011 , which claims priority to US Provisional Application No. 61 / 299,315, filed January 28, 2010. This application is also a continuation-in-part of US Application No. 13 / 208,459, filed August 12, 2011, which claims priority to US Provisional Application No. 61 / 373,711, filed August 13, 2010. This application further claims U.S. Provisional Application No. 61 / 614,994, filed March 23, 2012, U.S. Provisional Application No. 61 / 642,094, filed May 3, 2012, and U.S. Provisional Application No. 61 / 642,094, filed May 13, 2012. Priority of Provisional Application No. 61 / 646,337. The contents of all of the above applications are hereby incorporated by reference for all purposes. Background technique [0003] The preservation of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/30A61K47/48A61K9/20A61K9/48A61K9/14A23K10/18A23K10/20A23K10/30A23K20/00A23K20/10A23K20/147A23K20/158A23K20/163A23K20/184A23K20/189A23K20/195A23K40/10
CPCA61K9/1623A61K9/1652A61K9/1658A61K9/19A61K38/00A61K35/00A61K47/50A61K9/14A61K9/20A61K9/48A61K47/30A61K47/36A23K10/16A23L29/30A23P30/32A61K47/54C12N1/00C12N7/00
Inventor 莫迪·哈莱尔琼·唐特丽莎·赖斯金伯利·詹宁斯布莱恩·卡朋特罗杰·德鲁斯伊丽莎白·拉迪斯
Owner ADVANCED BIONUTRITION CORP
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