Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof

A technology of horse-faced fish and oxidized glue, which is applied to the preparation method of peptides, chemical instruments and methods, food preparation, etc., can solve the problems that high-activity fish anti-oxidation peptides have not been reported, and achieve significant anti-oxidation activity, safe and non-toxic Toxic and side effects, easy to digest and absorb

Active Publication Date: 2014-12-31
安徽盛美诺生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the applicant found that the preparation of antioxidant collagen peptides from horse-faced puffer fish skin by enzymatic hydrolysis technology is still in a blank stage, and the preparation of highly active fish meat antioxidant peptides using enzymatic hydrolysis products as materials and its applications have not been reported

Method used

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  • Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof
  • Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof
  • Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof

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Embodiment 1

[0029] 1) Fish skin pretreatment: mince the skin of the green-finned puffer fish, add isopropanol according to the ratio of solid to liquid 1 g:5 mL, soak at 4°C for 6 h, and change the isopropanol every 2 h to obtain Defatted fish skin: After the degreased fish skin is fully dried at 40°C, add 0.5 mol / L EDTA-2Na solution (pH 7.4) according to the ratio of solid to liquid 1 g:8 mL, soak at 4°C for 4 days, and replace EDTA-2Na once a day. 2Na solution to obtain degreased and decalcified fish skin; degreased and decalcified fish skin was fully dried at 40 °C and soaked in 0.1 mol / L NaOH solution at 4 °C for 6 h at a solid-liquid ratio of 1 g:15 mL, and replaced every 2 h. NaOH solution once to obtain degreased, decalcified and non-collagenized fish skin, fully dried at 40°C.

[0030] 2) Enzymatic hydrolysis: Add the degreased, decalcified and non-collagenous horse-faced puffer fish skin to the glycine-NaOH buffer (0.05 mol / L, pH 9.6) according to the ratio of solid to liquid 1 g...

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Abstract

The invention relates to a navodon septentrionalis fish-skin antioxidant collagen peptide and use thereof, and the navodon septentrionalis fish-skin antioxidant collagen peptide is characterized in that: the amino acid sequence is Phe-Ile-Gly-Pro-Trp, and the molecular weight is 618.75 Da ((M + H) + 619.63Da) given by ESI (electronic spray ion)-MS (mass spectrum) detection. The preparation method of the navodon septentrionalis fish-skin antioxidant collagen peptide includes navodon septentrionalis fish-skin pretreatment, enzymolysis, ultrafiltration and chromatography and other steps. The prepared antioxidative peptide Phe-Ile-Gly-Pro-Trp has good scavenging effect on DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, hydroxyl radical and superoxide anion free radical; at the same time, the Phe-Ile-Gly-Pro-Trp also shows good lipid peroxidation inhibition effect. The Phe-Ile-Gly-Pro-Trp has significant antioxidant activity, and can be used as medicines, health food or food additives.

Description

technical field [0001] The invention relates to a biologically active peptide and its preparation method and use, in particular to a horse-faced puffer fish skin antioxidant collagen peptide and its preparation method and use. Background technique [0002] Horse-faced puffer is a common fish in my country. The skin of horse-faced puffer is thick and hard, so after fishing, if time permits, the skin will be peeled on the spot, and its meat will be refrigerated in storage until it is eaten, and its skin will be thrown away on the spot, resulting in waste of resources and environmental pollution. At present, there have been researches on the use of horse-faced fish skin for the leather industry, the preparation of collagen, and the separation and purification of arginine and proline. Using horse-faced puffer fish skin as raw material and using enzymatic hydrolysis technology to develop collagen peptides will also lay a foundation for the high-value utilization of horse-faced p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61K38/08A61P39/06A23L1/29A23L1/305A23L33/18
Inventor 王斌王晓玲迟长凤王玉梅
Owner 安徽盛美诺生物技术有限公司
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